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α‐Amylase of the thermophilic actinomycete Thermomonospora vulgaris was partially purified. Maximal enzyme activity was obtained at 60 °C and pH 6.0. KM value was 1.4%. The effect of some metal salts on enzyme activity was studied. Enzyme activity was inhibited by KCN, EDTA, and iodoacetate....
A stable spheroplast type L‐form could be isolated by transferring 627 single colonies and 195 agar blocks with several colonies of unstable L‐forms of Proteus mirabilis D 52 on agar media without supplements of penicillin. The L‐form grows well on complex and synthetic agar media, however, it...
Resistomycin preferentially inhibits RNA synthesis in comparison to DNA and protein synthesis in intact bacterial cells. Studies with cell‐free systems have shown that the antibiotic interferes with DNA and RNA synthesis, while protein synthesis is inhibited to a much lesser extent. Detailed...
The utilization of n‐hexadecane by Candida lipolytica (strain 10) was studied with respect to the lipid content, phospholipid and fatty acid profiles resulting at various growth times. Thin layer chromatography of the lipid extracts showed quantitative changes in the different lipid classes. The...
Die Eigenschaften und der Einfluß von organischen Säuren und Nucleotiden auf die Aktivität der hoch‐ (ICD I) und niedermolekularen (ICD II) NADP+‐abhängigen Isocitratdehydrogenase aus Acinetobacter calcoaceticus werden untersucht.
Paromomycin was isolated from culture filtrates of Streptomyces albus var. metamycinus nov. var. after feeding the growing cultures with D‐glucose‐u‐14C. From the different incorporation rates conclusions concerning different features of the paromomycin biosynthesis (utilization of the carbon...
A temperature‐sensitive strain (HD 20) of Salmonella typhimurium is described. At restrictive temperature this strain shows an envelope alteration and a defect in division associated with an increase in cell diameter. On a shift to 42 °C there is residual division for ca. 30 min and then no...
Bei A. calcoaceticus 69‐V wird nach Ultraschallaufschluß im zellfreien Rohextrakt ein Pyridin‐nucleotid‐unabhängiges Enzym zur Oxydation von längerkettigen primären aliphatischen Alkoholen nachgewiesen. Als Testsubstrate wurden Hexanol und Octanol verwendet. Als Elektronen‐akzeptor kann...
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