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Utilization of various phosphonates as source for phosphorus by the glyphosate degrading strain Pseudomonas spec. GS was investigated. Metabolites of phosphonate degradation were characterized indicating the cleavage of the C—P‐bond as primary step of breakdown. The phosphonate...
Mycobacterium fortuitum NRRL B‐8119 transformes sterols into 9 alpha‐hydroxy‐androsta‐4‐ene‐3,17‐dione (9OH‐AD) at high efficieny. Cells strongly aggregate to the surface of the sterol particles forming stable agglomerates (“substrate immobilized cells”). Substrate uptake and product formation...
Properties of external and internal β‐glucosidases and glucoamylase of Arxula ademinivorans were compared. For the purification of enzymes, the C‐catabolite derepression mutant SBUB 724/41 was used. The following Km‐values for β‐glucosidases with cellobiose as substrate were measured: 14.3 mm...
Two‐dimensional gel electrophoresis was used to demonstrate the synthesis of approximately 65 (35S)‐methionine‐labelled soluble proteins between 0 and 10 min after the start of germination, of approximately 210 proteins at 10–20 min, and of approximately 260 proteins during vegetative growth of...
Twenty‐two species belonging to 11 genera of zoosporic fungi were collected from 10 freshwater sites and from five species of leeches on sesame and hemp seeds, maize grains and Pinus pollen grains as baits at 22°C. Dictyuchus, Achlya, Pythium, Allomyces and Saprolegnia were the most common...
A virus‐like particle (VLP) of 42 nm diameter was isolated from the lipolytic microorganism Geotrichum candidum. The VLP contains a linear double‐stranded RNA molecule of 1.70 μm in length. Proteins of VLP were studied by polyacryl amide gel electrophoresis.
A survey is given of possible solutions and open‐ended questions in the biotransformation of steroids (without side chain degradation of sterols) by using immobilized cells. The data of literature between 1975 and 1990 and results of preliminary experiments from the microbial, biochemical,...
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