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Preimplantation genetic diagnosis (PGD) is an important option for couples at risk of having children with β-globin mutations to avoid selective abortions of affected fetuses following prenatal diagnosis.
Studies indicating that the developmental competence of the human oocyte is influenced by the level of intrafollicular oxygen are described.
Our purpose was to review recent progress in the use of nuclear transfer technology to produce genetically identical mammals.
Chromosome abnormalities in sperm were studied by fluorescence in situ hybridization to determine the frequency and distribution of abnormalities in normal men and the effect of donor age on the frequency of abnormalities. Studies of chemotherapy and infertility patients assessed any...
Because allele dropout (ADO) is frequently observed in single-cell polymerase chain reaction analysis, it is important to develop a method for efficient detection of ADO, in order to avoid possible misdiagnosis in preimplantation diagnosis.
Although conventional polymerase chain reaction (PCR) was the first method used for sexing in preimplantation genetic diagnosis, fluorescent in situ hybridization (FISH) has become the method of choice. Recently two new techniques, primed in situ synthesis (PRINS) and fluorescent PCR,...
Purpose and Methods:
Prenatal diagnosis of fetal trisomies is usually performed by cytogenetic analysis from amniotic fluid. This requires lengthy laboratory procedures and high costs and is unsuitable for large-scale screening of pregnant women. An alternative method, which is rapid and...
Our purpose was to assess the effect of chromosomal mosaicism in cleavage-stage human embryos on the accuracy of single-cell analysis for preimplantation genetic diagnosis.
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