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Development of a Rapid Assimilable Organic Carbon Method for Water

Development of a Rapid Assimilable Organic Carbon Method for Water Development of a Rapid Assimilable Organic Carbon Method for Water Mark W. Le Chevallier 1 * , Nancy E. Shaw 1 , Louis A. Kaplan 2 and Thomas L. Bott 2 1 American Water Works Service Co., Inc., 1115 South Illinois Street, Belleville, Illinois 62220, and Stroud Water Research Center, Academy of Natural Sciences of Philadelphia, Avondale, Pennsylvania 19311-9516 2 ABSTRACT A rapid method for measurement of assimilable organic carbon (AOC) is proposed. The time needed to perform the assay is reduced by increasing the incubation temperature and increasing the inoculum density. The ATP luciferin-luciferase method quickly enumerates the test organisms without the need for plate count media or dilution bottles. There was no significant difference between AOC values determined with strain P17 for the ATP and plate count procedures. For strain NOX, the plate count procedure underestimated bacterial levels in some samples. Comparison of AOC values obtained by the Belleville laboratory (by the ATP technique) and the Stroud Water Research Center (by plate counts) showed that values were significantly correlated and not significantly different. The study concludes that the rapid AOC method can quickly determine the bacterial growth potential of water within 2 to 4 days. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

Development of a Rapid Assimilable Organic Carbon Method for Water

Development of a Rapid Assimilable Organic Carbon Method for Water

Applied and Environmental Microbiology , Volume 59 (5): 1526 – May 1, 1993

Abstract

Development of a Rapid Assimilable Organic Carbon Method for Water Mark W. Le Chevallier 1 * , Nancy E. Shaw 1 , Louis A. Kaplan 2 and Thomas L. Bott 2 1 American Water Works Service Co., Inc., 1115 South Illinois Street, Belleville, Illinois 62220, and Stroud Water Research Center, Academy of Natural Sciences of Philadelphia, Avondale, Pennsylvania 19311-9516 2 ABSTRACT A rapid method for measurement of assimilable organic carbon (AOC) is proposed. The time needed to perform the assay is reduced by increasing the incubation temperature and increasing the inoculum density. The ATP luciferin-luciferase method quickly enumerates the test organisms without the need for plate count media or dilution bottles. There was no significant difference between AOC values determined with strain P17 for the ATP and plate count procedures. For strain NOX, the plate count procedure underestimated bacterial levels in some samples. Comparison of AOC values obtained by the Belleville laboratory (by the ATP technique) and the Stroud Water Research Center (by plate counts) showed that values were significantly correlated and not significantly different. The study concludes that the rapid AOC method can quickly determine the bacterial growth potential of water within 2 to 4 days.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1993 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
Publisher site
See Article on Publisher Site

Abstract

Development of a Rapid Assimilable Organic Carbon Method for Water Mark W. Le Chevallier 1 * , Nancy E. Shaw 1 , Louis A. Kaplan 2 and Thomas L. Bott 2 1 American Water Works Service Co., Inc., 1115 South Illinois Street, Belleville, Illinois 62220, and Stroud Water Research Center, Academy of Natural Sciences of Philadelphia, Avondale, Pennsylvania 19311-9516 2 ABSTRACT A rapid method for measurement of assimilable organic carbon (AOC) is proposed. The time needed to perform the assay is reduced by increasing the incubation temperature and increasing the inoculum density. The ATP luciferin-luciferase method quickly enumerates the test organisms without the need for plate count media or dilution bottles. There was no significant difference between AOC values determined with strain P17 for the ATP and plate count procedures. For strain NOX, the plate count procedure underestimated bacterial levels in some samples. Comparison of AOC values obtained by the Belleville laboratory (by the ATP technique) and the Stroud Water Research Center (by plate counts) showed that values were significantly correlated and not significantly different. The study concludes that the rapid AOC method can quickly determine the bacterial growth potential of water within 2 to 4 days.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: May 1, 1993

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