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M Protein of the Group A StreptococcusBinds to the Seventh Short Consensus Repeat of Human Complement Factor H

M Protein of the Group A StreptococcusBinds to the Seventh Short Consensus Repeat of Human... M Protein of the Group A Streptococcus Binds to the Seventh Short Consensus Repeat of Human Complement Factor H Timothy K. Blackmore 1 , * , Vincent A. Fischetti 2 , Tania A. Sadlon 1 , Helena M. Ward 1 and David L. Gordon 1 Department of Microbiology and Infectious Diseases, Flinders University of South Australia and Flinders Medical Centre, Bedford Park, South Australia 5042, Australia, 1 and Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021 2 ABSTRACT Streptococcus pyogenes evades complement by binding the complement-regulatory protein factor H (fH) via the central conserved C-repeat region of M protein. However, the corresponding binding region within fH has not previously been precisely localized. fH is composed of 20 conserved modules called short consensus repeats (SCRs), each of which contains approximately 60 amino acids. A series of fH truncated and deletion mutants were prepared, and their interaction with M6 protein was examined. The M protein binding site was initially localized to SCRs 6 to 15 as demonstrated by ligand dot blotting, chemical cross-linking, and enzyme-linked immunosorbent assay. SCR 7 was then shown to contain the M protein binding site, as a construct consisting of the first seven SCRs bound M protein but a construct containing the first six SCRs did not bind. In addition, deletion of SCR 7 from full-length fH abolished binding to M protein. SCR 7 is known to contain a heparin binding domain, and binding of fH to M6 protein was almost totally inhibited in the presence of 400 U of heparin per ml. These results localize the M6 protein binding site of fH to SCR 7 and indicate that it is in close proximity to the heparin binding site. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Infection and Immunity American Society For Microbiology

M Protein of the Group A StreptococcusBinds to the Seventh Short Consensus Repeat of Human Complement Factor H

M Protein of the Group A StreptococcusBinds to the Seventh Short Consensus Repeat of Human Complement Factor H

Infection and Immunity , Volume 66 (4): 1427 – Apr 1, 1998

Abstract

M Protein of the Group A Streptococcus Binds to the Seventh Short Consensus Repeat of Human Complement Factor H Timothy K. Blackmore 1 , * , Vincent A. Fischetti 2 , Tania A. Sadlon 1 , Helena M. Ward 1 and David L. Gordon 1 Department of Microbiology and Infectious Diseases, Flinders University of South Australia and Flinders Medical Centre, Bedford Park, South Australia 5042, Australia, 1 and Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021 2 ABSTRACT Streptococcus pyogenes evades complement by binding the complement-regulatory protein factor H (fH) via the central conserved C-repeat region of M protein. However, the corresponding binding region within fH has not previously been precisely localized. fH is composed of 20 conserved modules called short consensus repeats (SCRs), each of which contains approximately 60 amino acids. A series of fH truncated and deletion mutants were prepared, and their interaction with M6 protein was examined. The M protein binding site was initially localized to SCRs 6 to 15 as demonstrated by ligand dot blotting, chemical cross-linking, and enzyme-linked immunosorbent assay. SCR 7 was then shown to contain the M protein binding site, as a construct consisting of the first seven SCRs bound M protein but a construct containing the first six SCRs did not bind. In addition, deletion of SCR 7 from full-length fH abolished binding to M protein. SCR 7 is known to contain a heparin binding domain, and binding of fH to M6 protein was almost totally inhibited in the presence of 400 U of heparin per ml. These results localize the M6 protein binding site of fH to SCR 7 and indicate that it is in close proximity to the heparin binding site.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1998 by the American society for Microbiology.
ISSN
0019-9567
eISSN
1098-5522
Publisher site
See Article on Publisher Site

Abstract

M Protein of the Group A Streptococcus Binds to the Seventh Short Consensus Repeat of Human Complement Factor H Timothy K. Blackmore 1 , * , Vincent A. Fischetti 2 , Tania A. Sadlon 1 , Helena M. Ward 1 and David L. Gordon 1 Department of Microbiology and Infectious Diseases, Flinders University of South Australia and Flinders Medical Centre, Bedford Park, South Australia 5042, Australia, 1 and Laboratory of Bacterial Pathogenesis and Immunology, The Rockefeller University, New York, New York 10021 2 ABSTRACT Streptococcus pyogenes evades complement by binding the complement-regulatory protein factor H (fH) via the central conserved C-repeat region of M protein. However, the corresponding binding region within fH has not previously been precisely localized. fH is composed of 20 conserved modules called short consensus repeats (SCRs), each of which contains approximately 60 amino acids. A series of fH truncated and deletion mutants were prepared, and their interaction with M6 protein was examined. The M protein binding site was initially localized to SCRs 6 to 15 as demonstrated by ligand dot blotting, chemical cross-linking, and enzyme-linked immunosorbent assay. SCR 7 was then shown to contain the M protein binding site, as a construct consisting of the first seven SCRs bound M protein but a construct containing the first six SCRs did not bind. In addition, deletion of SCR 7 from full-length fH abolished binding to M protein. SCR 7 is known to contain a heparin binding domain, and binding of fH to M6 protein was almost totally inhibited in the presence of 400 U of heparin per ml. These results localize the M6 protein binding site of fH to SCR 7 and indicate that it is in close proximity to the heparin binding site.

Journal

Infection and ImmunityAmerican Society For Microbiology

Published: Apr 1, 1998

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