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Minimal Requirements for Exponential Growth of Lactococcus lactis

Minimal Requirements for Exponential Growth of Lactococcus lactis Minimal Requirements for Exponential Growth of Lactococcus lactis Peter Ruhdal Jensen 1 and Karin Hammer 2 * 2 Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, 2 and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands ABSTRACT A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis , with a specific growth rate in batch culture of μ = 0.3 h -1 . With 19 amino acids added, the growth rate increased to μ = 0.7 h -1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Applied and Environmental Microbiology American Society For Microbiology

Minimal Requirements for Exponential Growth of Lactococcus lactis

Minimal Requirements for Exponential Growth of Lactococcus lactis

Applied and Environmental Microbiology , Volume 59 (12): 4363 – Dec 1, 1993

Abstract

Minimal Requirements for Exponential Growth of Lactococcus lactis Peter Ruhdal Jensen 1 and Karin Hammer 2 * 2 Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, 2 and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands ABSTRACT A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis , with a specific growth rate in batch culture of μ = 0.3 h -1 . With 19 amino acids added, the growth rate increased to μ = 0.7 h -1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

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Publisher
American Society For Microbiology
Copyright
Copyright © 1993 by the American society for Microbiology.
ISSN
0099-2240
eISSN
1098-5336
Publisher site
See Article on Publisher Site

Abstract

Minimal Requirements for Exponential Growth of Lactococcus lactis Peter Ruhdal Jensen 1 and Karin Hammer 2 * 2 Department of Microbiology, Technical University of Denmark, DK-2800 Lyngby, Denmark, 2 and Division of Molecular Biology, Netherlands Cancer Institute H5, NL-1066-CX, Amsterdam, The Netherlands ABSTRACT A minimal growth medium containing glucose, acetate, vitamins, and eight amino acids allowed for growth of Lactococcus lactis subsp. lactis , with a specific growth rate in batch culture of μ = 0.3 h -1 . With 19 amino acids added, the growth rate increased to μ = 0.7 h -1 and the exponential growth phase proceeded until high cell concentrations were reached. We show that morpholinepropanesulfonic acid (MOPS) is a suitable buffer for L. lactis and may be applied in high concentrations.

Journal

Applied and Environmental MicrobiologyAmerican Society For Microbiology

Published: Dec 1, 1993

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