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The Cholinergic Receptor Protein in Its Membrane Environment

The Cholinergic Receptor Protein in Its Membrane Environment The cholinergic receptor protein from fish electric organ is the first pharmacological receptor with a well-characterized physiological function to have been purified and isolated in milligram quantities (I-II). Nevertheless, the mechanism by which this regulatory protein (12) controls membrane permeability and the binding of acetyl­ choline remains largely unknown. Therefore, we focus this review on the receptor protein in its membrane environment. The electric organs from Electrophorus. a fresh water Teleost, and Torpedo. a sea water Elasmobranch, are still the most convenient systems for parallel electrophysiological and biochemical studies. We devote this review to work done on single cell preparations (electroplaques) and on membrane suspensions prepared from these organs. We often refer to ligands which, in addition to the typical nicotinic ligands, have been particularly useful for the selective labeling of cholinergic receptor sites: the snake a-toxins (13, 14) and, affinity labeling reagents (15, 16). Several reviewers have discussed the anatomy and electrophysiology of the electric organs (17, 18) as well as the characterization, isolation, and purification of the cholinergic receptor from these organs (16, 19, 20). 'Experimental work from this laboratory was supported by funds from the Centre National nique. the College de France, the Commissariat a http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Annual Review of Pharmacology and Toxicology Annual Reviews

The Cholinergic Receptor Protein in Its Membrane Environment

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Publisher
Annual Reviews
Copyright
Copyright 1975 Annual Reviews. All rights reserved
Subject
Review Articles
ISSN
0362-1642
eISSN
1545-4304
DOI
10.1146/annurev.pa.15.040175.000503
pmid
1096798
Publisher site
See Article on Publisher Site

Abstract

The cholinergic receptor protein from fish electric organ is the first pharmacological receptor with a well-characterized physiological function to have been purified and isolated in milligram quantities (I-II). Nevertheless, the mechanism by which this regulatory protein (12) controls membrane permeability and the binding of acetyl­ choline remains largely unknown. Therefore, we focus this review on the receptor protein in its membrane environment. The electric organs from Electrophorus. a fresh water Teleost, and Torpedo. a sea water Elasmobranch, are still the most convenient systems for parallel electrophysiological and biochemical studies. We devote this review to work done on single cell preparations (electroplaques) and on membrane suspensions prepared from these organs. We often refer to ligands which, in addition to the typical nicotinic ligands, have been particularly useful for the selective labeling of cholinergic receptor sites: the snake a-toxins (13, 14) and, affinity labeling reagents (15, 16). Several reviewers have discussed the anatomy and electrophysiology of the electric organs (17, 18) as well as the characterization, isolation, and purification of the cholinergic receptor from these organs (16, 19, 20). 'Experimental work from this laboratory was supported by funds from the Centre National nique. the College de France, the Commissariat a

Journal

Annual Review of Pharmacology and Toxicology – Annual Reviews

Published: Apr 1, 1975

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