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Electrochemical immunosensor for a protein biomarker based on the formation of Prussian blue with magnetic nanoparticle

Electrochemical immunosensor for a protein biomarker based on the formation of Prussian blue with... <jats:p>An electrochemical immunosensor for the detection of protein biomarkers is reported herein based on using magnetic nanoparticles (MNs) as labels. Reaction of potassium ferrocyanide with MNs leads to<jats:italic> in situ</jats:italic> formation of Prussian blue (PB) on the MN surface, generating an electrochemical current. A carcinoembryonic antigen (CEA) was chosen as a model protein biomarker to test the performance of the immunosensor. The immunosensor was prepared based on a traditional sandwich-type structure. A high concentration of CEA increased the number of MNs captured on the electrode, which increased the amount of PB produced, thus increasing the electrochemical current intensity. The current intensity linearly increased with the concentration of CEA in the range from 0.05 to 10 ng/mL. The potential clinical applicability of this immunosensor was demonstrated by CEA detection in human serum samples.</jats:p> http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Materials Express CrossRef

Electrochemical immunosensor for a protein biomarker based on the formation of Prussian blue with magnetic nanoparticle

Materials Express , Volume 10 (2): 278-282 – Feb 1, 2020

Electrochemical immunosensor for a protein biomarker based on the formation of Prussian blue with magnetic nanoparticle


Abstract

<jats:p>An electrochemical immunosensor for the detection of protein biomarkers is reported herein based on using magnetic nanoparticles (MNs) as labels. Reaction of potassium ferrocyanide with MNs leads to<jats:italic> in situ</jats:italic> formation of Prussian blue (PB) on the MN surface, generating an electrochemical
current. A carcinoembryonic antigen (CEA) was chosen as a model protein biomarker to test the performance of the immunosensor. The immunosensor was prepared based on a traditional sandwich-type structure. A high concentration of CEA increased the number of MNs captured on the electrode, which
increased the amount of PB produced, thus increasing the electrochemical current intensity. The current intensity linearly increased with the concentration of CEA in the range from 0.05 to 10 ng/mL. The potential clinical applicability of this immunosensor was demonstrated by CEA detection
in human serum samples.</jats:p>

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Publisher
CrossRef
ISSN
2158-5849
DOI
10.1166/mex.2020.1635
Publisher site
See Article on Publisher Site

Abstract

<jats:p>An electrochemical immunosensor for the detection of protein biomarkers is reported herein based on using magnetic nanoparticles (MNs) as labels. Reaction of potassium ferrocyanide with MNs leads to<jats:italic> in situ</jats:italic> formation of Prussian blue (PB) on the MN surface, generating an electrochemical current. A carcinoembryonic antigen (CEA) was chosen as a model protein biomarker to test the performance of the immunosensor. The immunosensor was prepared based on a traditional sandwich-type structure. A high concentration of CEA increased the number of MNs captured on the electrode, which increased the amount of PB produced, thus increasing the electrochemical current intensity. The current intensity linearly increased with the concentration of CEA in the range from 0.05 to 10 ng/mL. The potential clinical applicability of this immunosensor was demonstrated by CEA detection in human serum samples.</jats:p>

Journal

Materials ExpressCrossRef

Published: Feb 1, 2020

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