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Anti- Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells

Anti- Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells Abstract Anisakiosis is nowadays one of the nematodoses more prevalent in Spain, with rates that oscillate between 0.43% in Galicia (N.W. Spain), and 15.7% and 22.1% in inland and southern regions, respectively. Likewise, it has been proved that Anisakis larvae have developed mechanisms to modulate the dichotomy of the host immune response for their own benefit. The experimental hypothesis of the present study was that Anisakis sp. larval products can be mediators of immune suppression and induce changes on the populations of αβ+ and γδ+ T cells. In the present study we determined the levels of anti- Anisakis antibodies in the serum of healthy people, and their relationship with the B and T cell subsets. Levels of anti- Anisakis antibodies (Ig’s, IgG, IgM, IgA and IgE) were measured by ELISA, while B and T cell subsets were studied by flow cytometry. Cells were labelled with monoclonal antibodies against CD45, CD4, CD8, CD56, CD3, CD19, TCRαβ and TCRγδ. All the specific isotypes studied were negatively correlated with NKT cell rates with the exception of IgG. A previous contact with Anisakis was related to a decrease in CD56+αβ+ and all γδ+ T cell subsets. The CD3+γδ+ population was lower in the group of subjects that showed IgA anti- Anisakis . We observed an inverse correlation among αβ-γδ NKT cells and anti- Anisakis sp. antibodies. CD3+CD56+ cells showed a significant decrease in the group of anti- Anisakis positive subjects. This fact was especially significant with CD3+CD56+γδ+ cells in the case of the anti- Anisakis IgA positive group. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Parasitologica de Gruyter

Anti- Anisakis sp. antibodies in serum of healthy subjects. Relationship with αβ and γδ T cells

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Publisher
de Gruyter
Copyright
Copyright © 2017 by the
ISSN
1230-2821
eISSN
1896-1851
DOI
10.1515/ap-2017-0011
pmid
28030343
Publisher site
See Article on Publisher Site

Abstract

Abstract Anisakiosis is nowadays one of the nematodoses more prevalent in Spain, with rates that oscillate between 0.43% in Galicia (N.W. Spain), and 15.7% and 22.1% in inland and southern regions, respectively. Likewise, it has been proved that Anisakis larvae have developed mechanisms to modulate the dichotomy of the host immune response for their own benefit. The experimental hypothesis of the present study was that Anisakis sp. larval products can be mediators of immune suppression and induce changes on the populations of αβ+ and γδ+ T cells. In the present study we determined the levels of anti- Anisakis antibodies in the serum of healthy people, and their relationship with the B and T cell subsets. Levels of anti- Anisakis antibodies (Ig’s, IgG, IgM, IgA and IgE) were measured by ELISA, while B and T cell subsets were studied by flow cytometry. Cells were labelled with monoclonal antibodies against CD45, CD4, CD8, CD56, CD3, CD19, TCRαβ and TCRγδ. All the specific isotypes studied were negatively correlated with NKT cell rates with the exception of IgG. A previous contact with Anisakis was related to a decrease in CD56+αβ+ and all γδ+ T cell subsets. The CD3+γδ+ population was lower in the group of subjects that showed IgA anti- Anisakis . We observed an inverse correlation among αβ-γδ NKT cells and anti- Anisakis sp. antibodies. CD3+CD56+ cells showed a significant decrease in the group of anti- Anisakis positive subjects. This fact was especially significant with CD3+CD56+γδ+ cells in the case of the anti- Anisakis IgA positive group.

Journal

Acta Parasitologica – de Gruyter

Published: Mar 1, 2017

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