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Optimization of enzymatic synthesis of ethyl hexanoate in a solvent free system using response surface methodology (RSM)

Optimization of enzymatic synthesis of ethyl hexanoate in a solvent free system using response... AbstractThe present paper demonstrates application of biocatalysis to the synthesis of ethyl hexanoate, i.e. pineapple flavour ester, in a solvent free system. In order to evaluate the effect of various process parameters on reaction conversion, response surface methodology (RSM) complemented by central composite design (CCD) was employed. A maximum conversion of 88.57% was obtained while changing one factor at a time, at optimum conditions of temperature (50 °C), enzyme dose (2%), molar ratio acid to alcohol (1:3), speed of agitation 250 rpm and reaction time of 120 min. Based on this RSM study, the optimum predicted conditions were: 1:3.39 alcohol to acid ratio, 2.35% enzyme loading and 48.83 oC, for a predicted conversion of 90.99%. The activation energy for the enzymatic esterification was determined and calculated to be 25.76 kJ/mol. The positive values of Gibbs-free energy (ΔG), enthalpy (ΔH) and negative value of entropy (ΔS) revealed that the esterification reaction was non-spontaneous and an endothermic reaction. The reaction seems to follow bi-substrate Ping Pong Bi Bi mechanism with inhibition by both substrates. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biocatalysis de Gruyter

Optimization of enzymatic synthesis of ethyl hexanoate in a solvent free system using response surface methodology (RSM)

Gawas, Sarita D.; Lokanath, Nidya; Rathod, Virendra K.
Biocatalysis , Volume 4 (1): 13 – May 17, 2018
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Publisher
de Gruyter
Copyright
© 2018
ISSN
2353-1746
eISSN
2353-1746
DOI
10.1515/boca-2018-0002
Publisher site
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Abstract

AbstractThe present paper demonstrates application of biocatalysis to the synthesis of ethyl hexanoate, i.e. pineapple flavour ester, in a solvent free system. In order to evaluate the effect of various process parameters on reaction conversion, response surface methodology (RSM) complemented by central composite design (CCD) was employed. A maximum conversion of 88.57% was obtained while changing one factor at a time, at optimum conditions of temperature (50 °C), enzyme dose (2%), molar ratio acid to alcohol (1:3), speed of agitation 250 rpm and reaction time of 120 min. Based on this RSM study, the optimum predicted conditions were: 1:3.39 alcohol to acid ratio, 2.35% enzyme loading and 48.83 oC, for a predicted conversion of 90.99%. The activation energy for the enzymatic esterification was determined and calculated to be 25.76 kJ/mol. The positive values of Gibbs-free energy (ΔG), enthalpy (ΔH) and negative value of entropy (ΔS) revealed that the esterification reaction was non-spontaneous and an endothermic reaction. The reaction seems to follow bi-substrate Ping Pong Bi Bi mechanism with inhibition by both substrates.

Journal

Biocatalysis – de Gruyter

Published: May 17, 2018

References

  • esterification of docosahexaenoic acid in an organic solvent - free system with immobilized Candida antarctica lipase
    Shimada
  • Enzymatic synthesis of ethyl hexanoate by transesterification
    Chowdary
  • Enhanced biocatalytic activity of immobilized Pseudomonas cepacia lipase under sonicated condition
    Badgujar
  • Solvent Free Lipase Catalysed Synthesis of Optimization and Kinetic Studies
    Gawas
  • a Enzyme inactivation by ethanol and development of a kinetic model for thermophilic simultaneous saccharification and fermentation at with Thermoanaerobacterium saccharolyticum
    Podkaminer
  • Investigations in sono - enzymatic degradation of ibuprofen
    Chakma
  • Computational Approach to Solvent - Free Synthesis of Ethyl Oleate Using Candida rugosa and Candida antarctica Activation and Substrate Ethanol Acid Adsorption
    Foresti
  • of ethyl butyrate using immobilized lipase : a statistical approach
    Rodriguez
  • Optimization of pineapple flavour synthesis by esterification catalysed by immobilized lipase from Rhizomucor miehei Flavour
    Lorenzoni
  • Esterification by immobilized lipase in solvent - free media : Kinetic and thermodynamic arguments
    Sandoval
  • Modelling and optimization of ethyl butyrate production catalysed by Rhizopus oryzae lipase
    Grosso
  • Highly efficient synthesis of ethyl hexanoate catalyzed by CALB - displaying Saccharomyces cerevisiae whole - cells in non - aqueous phase
    Han
  • Esterification in organic solvents by lipase immobilized in polymer of PVA - alginate - boric acid
    Dave
  • Solvent - free geranyl oleate production by enzymatic esterification
    Paroul
  • a Assessment of process variables on - ethylhexyl palmitate production using as catalyst in a solvent - free system
    Richetti
  • Kinetics of Esterification of Palmitic acid with Ethanol - Optimization Using Statistical Design of Experiments
    Beula
  • Thermodynamic analysis of the kinetics reactions of the production of FAME and FAEE using as catalyst
    Gonzales
  • Heterogeneous catalysis in esterification reactions : preparation of phenethyl acetate and cyclohexyl acetate by using a variety of solid acidic catalysts
    Yadav
  • Application of Box - Behnken design and response surface methodology for modeling of some Turkish coals
    Aslan
  • isoascorbyl palmitate : lipase - catalyzed synthesis structural characterization and process optimization using response surface methodology
    Sun
  • Response surface methodology as a tool for optimization in analytical chemistry
    Bezerra
  • Optimization of the enzymatic production of isoamyl acetate with from Candida antarctica
    Guvenc
  • catalyzed synthesis of cinnamyl acetate via transesterification in non - aqueous medium
    Yadav
  • The potential use of lipases in the production of fatty acid derivatives for the food and nutraceutical industries
    Ferreira
  • Intensification of enzymatic synthesis of propylene glycol monolaurate from propanediol and lauric acid under microwave irradiation : Kinetics of forward and reverse reactions
    Yadav
  • of ethyl esters of short - chain fatty acids using whole - cell lipase from Rhizopus chinesis in non - aqueous phase
    Xu
  • potential biocatalyst for the synthesis of valuable flavour and fragrance ester compounds Flavour
    Dhake
  • Production of flavor esters catalyzed by CALB - displaying Pichia pastoris whole - cells in a batch reactor
    Jin
  • Biodiesel production using waste frying oil Waste
    Charpe
  • Synthesis of isobutyl propionate using immobilized lipase in a solvent free system : Optimization and kinetic studies
    Kuperkar
  • Loss on lipase - catalyzed reactions in ultrasound - assisted systems
    Lerin
  • Optimization of Enzymatic Synthesis of Acetate Fruit Flavor - Effect of the Support on the Properties of
    Corradini
  • Application of central composite rotatable design to lipase catalysed synthesis of cresyl acetate
    Manohar
  • Kinetic and thermodynamic investigation of enzymatic ascorbyl acetate synthesis
    Zhang
  • The dependence of enzyme activity on temperature : determination and validation of parameters
    Peterson