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/lp/de-gruyter/qualitative-and-quantitative-hplc-elsd-esi-ms-analysis-of-steroidal-7sNJJAbnUV
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- Publisher
- de Gruyter
- Copyright
- © 2020 Barbara Król-Kogus et al., published by Sciendo
- ISSN
- 1846-9558
- eISSN
- 1846-9558
- DOI
- 10.2478/acph-2020-0013
- Publisher site
- See Article on Publisher Site
Abstract
AbstractFenugreek seeds are known as a source of various compounds, the most common of which are steroidal saponins. However, despite the growing interest in this plant material as a healing agent, spice and dietary supplement ingredient, the composition of Polish fenugreek seeds remains unknown. Therefore, the steroidal saponin complex in the seeds of T. foenum-graecum cultivated in Poland was qualitatively and quantitatively analyzed by the HPLC-ELSDESI-MS method. Two C-18 columns connected in series were used for the first time in analysis of fenugreek saponins and ELS detector parameters were optimized. A total of 26 furostanol saponins were revealed, of which 24 were tentatively identified. The HPLC-ELSD method developed for quantitative analysis was preliminarily validated and the determined amount of steroidal saponins in Polish fenugreek seeds was 0.14 %.
Journal
Acta Pharmaceutica – de Gruyter
Published: Mar 1, 2020
Keywords: fenugreek seeds; saponins; HPLC-ELSD-ESI-MS; qualitative and quantitative analysis