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TLC-densitometric analysis of allantoin in Symphytum officinale L. roots

TLC-densitometric analysis of allantoin in Symphytum officinale L. roots AbstractA TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/water 80:20 (V/V) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer, and was in agreement with literature reports. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Pharmaceutica de Gruyter

TLC-densitometric analysis of allantoin in Symphytum officinale L. roots

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References (14)

Publisher
de Gruyter
Copyright
© 2020 Katarzyna Kimel et al., published by Sciendo
ISSN
1846-9558
eISSN
1846-9558
DOI
10.2478/acph-2020-0014
Publisher site
See Article on Publisher Site

Abstract

AbstractA TLC-densitometric method for determination of allantoin in Symphytum officinale root was developed. Densitometric quantification of allantoin was carried out on TLC Si60 plates with butanol-50 % methanol/formic acid, 66.5:33.2:0.3 (V/V/V) as developing solvent, at a wavelength of 190 nm. The method was preliminarily validated in terms of specificity, linearity, precision, limit of detection, limit of quantification, recovery and robustness. The results of TLC quantification were compared with HPLC analysis carried out on a HILIC Luna NH2 100A column, with mobile phase consisting of acetonitrile/water 80:20 (V/V) and UV detection at 190 and 210 nm. Allantoin content was determined in two herbal products and it varied from 0.94 to 2.09 %, depending on the producer, and was in agreement with literature reports.

Journal

Acta Pharmaceuticade Gruyter

Published: Mar 1, 2020

Keywords: allantoin; Symphytum officinale; densitometry; TLC; HPLC

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