Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

A Method for Quantifying Synchrony in Testes of Rats Treated with Vitamin A Deprivation and Readministration

A Method for Quantifying Synchrony in Testes of Rats Treated with Vitamin A Deprivation and... Abstract Using a variation of a previously published method for manipulating vitamin A levels, we obtained synchronized rat testes and determined the frequency of stages of the seminiferous epithelium in each rat. In this study, we have demonstrated a method for quantitative analysis of the synchrony. The degree of synchronization was expressed as a fraction of the cycle of the seminiferous epithelium, and thus in terms not influenced by the different durations of the stages of this cycle. The median stage about which the tubules were synchronized was calculated. This method may be used to compare the effects of different synchronizing treatments, which may be subtle, and to study various aspects of spermatogenesis in the synchronized testes. For example, the duration of the cycle of the seminiferous epithelium in synchronized testes is estimated to be 12.5 days. This content is only available as a PDF. Author notes 1 Support for this research was provided by Grant HD-16843 and by Grant CA-17364 from the National Institutes of Health. Copyright © 1990 by The Society for the Study of Reproduction http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biology of Reproduction Oxford University Press

A Method for Quantifying Synchrony in Testes of Rats Treated with Vitamin A Deprivation and Readministration

Loading next page...
 
/lp/oxford-university-press/a-method-for-quantifying-synchrony-in-testes-of-rats-treated-with-Jxv1fbgdO6

References (10)

Publisher
Oxford University Press
Copyright
Copyright © 1990 by The Society for the Study of Reproduction
ISSN
0006-3363
eISSN
1529-7268
DOI
10.1095/biolreprod42.3.424
Publisher site
See Article on Publisher Site

Abstract

Abstract Using a variation of a previously published method for manipulating vitamin A levels, we obtained synchronized rat testes and determined the frequency of stages of the seminiferous epithelium in each rat. In this study, we have demonstrated a method for quantitative analysis of the synchrony. The degree of synchronization was expressed as a fraction of the cycle of the seminiferous epithelium, and thus in terms not influenced by the different durations of the stages of this cycle. The median stage about which the tubules were synchronized was calculated. This method may be used to compare the effects of different synchronizing treatments, which may be subtle, and to study various aspects of spermatogenesis in the synchronized testes. For example, the duration of the cycle of the seminiferous epithelium in synchronized testes is estimated to be 12.5 days. This content is only available as a PDF. Author notes 1 Support for this research was provided by Grant HD-16843 and by Grant CA-17364 from the National Institutes of Health. Copyright © 1990 by The Society for the Study of Reproduction

Journal

Biology of ReproductionOxford University Press

Published: Mar 1, 1990

There are no references for this article.