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Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1

Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1 AbstractSpermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>1014-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Biology of Reproduction Oxford University Press

Long-Term Proliferation in Culture and Germline Transmission of Mouse Male Germline Stem Cells1

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References (37)

Publisher
Oxford University Press
Copyright
© 2003 by the Society for the Study of Reproduction, Inc.
ISSN
0006-3363
eISSN
1529-7268
DOI
10.1095/biolreprod.103.017012
pmid
12700182
Publisher site
See Article on Publisher Site

Abstract

AbstractSpermatogenesis is a complex process that originates in a small population of spermatogonial stem cells. Here we report the in vitro culture of spermatogonial stem cells that proliferate for long periods of time. In the presence of glial cell line-derived neurotrophic factor, epidermal growth factor, basic fibroblast growth factor, and leukemia inhibitory factor, gonocytes isolated from neonatal mouse testis proliferated over a 5-month period (>1014-fold) and restored fertility to congenitally infertile recipient mice following transplantation into seminiferous tubules. Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine.

Journal

Biology of ReproductionOxford University Press

Published: Aug 1, 2003

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