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NEONATAL GONOCYTES CO-CULTURED WITH SERTOLI CELLS ON A LAMININ-CONTAINING MATRIX RESUME MITOSIS AND ELONGATE

NEONATAL GONOCYTES CO-CULTURED WITH SERTOLI CELLS ON A LAMININ-CONTAINING MATRIX RESUME MITOSIS... We co-cultured gonocytes and Sertoli cells isolated on the day of birth and observed the appearance, 1 and 3 days after the start of culture, of gonocytes that had developed cellular processes and that were labeled by [3H] thymidine, respectively. These events occurred in the absence of hormones, etc. and with a time course very similar to that seen in vivo. In other incubations, we found that the presence of laminin in the underlying substrate was critical in promoting proliferation and elongation of gonocytes. These observations strongly suggest that interactions between gonocytes and other testicular cells/factors in the co-cultures promote maturation of these germ cells in vitro, and thus provide new evidence to support the concept that paracrine mechanisms are important during testicular development as well as in adults. This content is only available as a PDF. Copyright © 1991 by The Endocrine Society http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Endocrinology Oxford University Press

NEONATAL GONOCYTES CO-CULTURED WITH SERTOLI CELLS ON A LAMININ-CONTAINING MATRIX RESUME MITOSIS AND ELONGATE

Endocrinology , Volume 129 (2) – Aug 1, 1991

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References (11)

Publisher
Oxford University Press
Copyright
Copyright © 1991 by The Endocrine Society
ISSN
0013-7227
eISSN
1945-7170
DOI
10.1210/endo-129-2-1119
Publisher site
See Article on Publisher Site

Abstract

We co-cultured gonocytes and Sertoli cells isolated on the day of birth and observed the appearance, 1 and 3 days after the start of culture, of gonocytes that had developed cellular processes and that were labeled by [3H] thymidine, respectively. These events occurred in the absence of hormones, etc. and with a time course very similar to that seen in vivo. In other incubations, we found that the presence of laminin in the underlying substrate was critical in promoting proliferation and elongation of gonocytes. These observations strongly suggest that interactions between gonocytes and other testicular cells/factors in the co-cultures promote maturation of these germ cells in vitro, and thus provide new evidence to support the concept that paracrine mechanisms are important during testicular development as well as in adults. This content is only available as a PDF. Copyright © 1991 by The Endocrine Society

Journal

EndocrinologyOxford University Press

Published: Aug 1, 1991

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