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A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES

A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES Ins thue exheussivelv ussed Koelle ;onsd Fniederuwald are added in order, wills stirninug betweems each histociueuusbcal nuetisod Ion cisolinsestersose, amid its addition: 0.5 mssl 0.1 M sodiuuss citrate, 1 usul 30 several ns(odificat ions (see Pearse, Histocheun istry, mM CuSO4, 1 nub water, ant! I nul 5 caM potassium 2moded., p. 478, Little, Brown & Co., Bostons, 1960), fernicyanide. ‘rise stock soluntiomus keep ins the cold thse tiuiociso)biose ester used as substrate is hydro- for several weeks. The fiusal inucubsohioms usuedium iysed by cholimsestenosse, oond the liberated hhio- is clear, greenish ins color, sonstl is stable for Iuouirs. cisohinue is captured h)\ Cu icns, pnecipitatirug sos If esenine sulfate is used as somu ioshibihcor, it is added colorless copper thiocisolbuse. The latter is con- as 1 iusl of a freshly prepotred 1 .11 soolutions after vented to broot-nisis CuS by treatiuseust ot-iths yellow the copper sulfate, replacinsg hue 1 ussl oof water. aissosuonununsu sulfide. Voonious steps irs thse nusethod Above pH 7.0 copper camsosot be kept in solution, hsove- bee-mu criticized (Ion discussion see Pearse, unsless at least 3 toM citrate is preseost, ui-hsichu does not allow for sufficiently nsopid loommusations ccl cop- ibid.). Lsorge useedle-like (‘rystals (occur l)articU- http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Histochemistry & Cytochemistry SAGE

A "DIRECT-COLORING" THIOCHOLINE METHOD FOR CHOLINESTERASES

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Publisher
SAGE
Copyright
1964, THE WILLIAMS & WILKINS COMPANY
ISSN
0022-1554
eISSN
1551-5044
DOI
10.1177/12.3.219
pmid
14187330
Publisher site
See Article on Publisher Site

Abstract

Ins thue exheussivelv ussed Koelle ;onsd Fniederuwald are added in order, wills stirninug betweems each histociueuusbcal nuetisod Ion cisolinsestersose, amid its addition: 0.5 mssl 0.1 M sodiuuss citrate, 1 usul 30 several ns(odificat ions (see Pearse, Histocheun istry, mM CuSO4, 1 nub water, ant! I nul 5 caM potassium 2moded., p. 478, Little, Brown & Co., Bostons, 1960), fernicyanide. ‘rise stock soluntiomus keep ins the cold thse tiuiociso)biose ester used as substrate is hydro- for several weeks. The fiusal inucubsohioms usuedium iysed by cholimsestenosse, oond the liberated hhio- is clear, greenish ins color, sonstl is stable for Iuouirs. cisohinue is captured h)\ Cu icns, pnecipitatirug sos If esenine sulfate is used as somu ioshibihcor, it is added colorless copper thiocisolbuse. The latter is con- as 1 iusl of a freshly prepotred 1 .11 soolutions after vented to broot-nisis CuS by treatiuseust ot-iths yellow the copper sulfate, replacinsg hue 1 ussl oof water. aissosuonununsu sulfide. Voonious steps irs thse nusethod Above pH 7.0 copper camsosot be kept in solution, hsove- bee-mu criticized (Ion discussion see Pearse, unsless at least 3 toM citrate is preseost, ui-hsichu does not allow for sufficiently nsopid loommusations ccl cop- ibid.). Lsorge useedle-like (‘rystals (occur l)articU-

Journal

Journal of Histochemistry & CytochemistrySAGE

Published: Mar 1, 1964

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