Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

CYTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE BY THE USE OF A NEW p-NITROPHENYL SUBSTITUTED DITETRAZOLE

CYTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE BY THE USE OF A NEW p-NITROPHENYL... 1. A new p-nitrophenyl substituted ditetrazole, 2,2'-di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene) ditetrazolium chloride (Nitro-BT), is introduced, which possesses most of the properties of an ideal reagent for histochemical purposes. It permits the cytochemical visualization of the sites of enzymic activity in tissue sections from 2-8 µ thick, under aerobic conditions, after a relatively short incubation period.2. A comparison was made of the various tetrazoles in common use with several new p-nitro substituted analogues of these agents.3. A survey was presented of the enzyme distribution in the major tissues of the rat and dog. Evidence for the reliability of cytochemical localization is provided by illustrative material in selected tissues.4. The new tetrazole can be employed for the diphosphopyridine nucleotide and triphosphopyridine nucleotide diaphorase systems as well as for the succinic dehydrogenase system by substituting Nitro-BT for blue tetrazolium (BT) in the incubation media. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Histochemistry & Cytochemistry SAGE

CYTOCHEMICAL DEMONSTRATION OF SUCCINIC DEHYDROGENASE BY THE USE OF A NEW p-NITROPHENYL SUBSTITUTED DITETRAZOLE

Loading next page...
 
/lp/sage/cytochemical-demonstration-of-succinic-dehydrogenase-by-the-use-of-a-uCf8G0oMtH

References (18)

Publisher
SAGE
Copyright
1957, THE WILLIAMS & WILKINS COMPANY
ISSN
0022-1554
eISSN
1551-5044
DOI
10.1177/5.4.420
pmid
13463314
Publisher site
See Article on Publisher Site

Abstract

1. A new p-nitrophenyl substituted ditetrazole, 2,2'-di-p-nitrophenyl-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-biphenylene) ditetrazolium chloride (Nitro-BT), is introduced, which possesses most of the properties of an ideal reagent for histochemical purposes. It permits the cytochemical visualization of the sites of enzymic activity in tissue sections from 2-8 µ thick, under aerobic conditions, after a relatively short incubation period.2. A comparison was made of the various tetrazoles in common use with several new p-nitro substituted analogues of these agents.3. A survey was presented of the enzyme distribution in the major tissues of the rat and dog. Evidence for the reliability of cytochemical localization is provided by illustrative material in selected tissues.4. The new tetrazole can be employed for the diphosphopyridine nucleotide and triphosphopyridine nucleotide diaphorase systems as well as for the succinic dehydrogenase system by substituting Nitro-BT for blue tetrazolium (BT) in the incubation media.

Journal

Journal of Histochemistry & CytochemistrySAGE

Published: Jul 1, 1957

There are no references for this article.