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Identification and Characterisation of the Salt Tolerant Phosphate-Solubilising Bacterial Isolates for Enhancing Soil Fertility

Identification and Characterisation of the Salt Tolerant Phosphate-Solubilising Bacterial... In this study, 13 bacteria were isolated from the agricultural soils of Kyaukse city, Myanmar, and out of these isolates, two bacteria isolates viz. JU-24 and JU-33 are found to be potent phosphate (P) solubilisers and are selected for further studies. These two isolates are identified as Bacillus megaterium based on the 16S rRNA gene sequence. When the growth rates of these two isolates were tested for salt tolerance, they could grow well in media with NaCl concentrations of 3%, 6% and 9%. For 0% and 3% NaCl concentrations, JU-33 (84.37 mg/L for 0% NaCl and 130.36 mg/L for 3% NaCl) showed higher P-solubilising efficiency than JU-24 (73.29 mg/L for 0% NaCl and 87.42 mg/L for 3% NaCl) for both NaCl concentrations when inoculated in Ca3(PO4)2 containing liquid medium. In media containing AlPO4 and FePO4 without NaCl, the P-solubilising activity of JU-24 is higher than JU-33 for these two substrates. Moreover, these strains showed noticeable levels of P solubilisation activity in the presence of various carbon sources indicating high P-solubilising efficacy. The soil inoculation experiment revealed that inoculating the experimental soil with JU-33 resulted in an obvious increase in available P, which increased from 13.08 mg/L in the non-inoculated soil to 18.40 mg/L in the inoculated soil with the bacterial isolate, JU-33. This study clearly showed that JU-24 and JU-33 isolates could be used as biofertilizers in ecological agricultural systems and may help to sustain environmental health and soil productivity. The use of biofertilizers can reduce the use of expensive chemical fertilisers, reducing planting costs and improving soil fertility through long-term use. Biofertilizers can help solve the problem of feeding an increasing global population at a time when agriculture is facing various environmental stresses and are affordable for low-income farmers. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Asia-Pacific Journal of Rural Development SAGE

Identification and Characterisation of the Salt Tolerant Phosphate-Solubilising Bacterial Isolates for Enhancing Soil Fertility

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Publisher
SAGE
Copyright
© 2022 Centre on Integrated Rural Development for Asia and the Pacific
ISSN
1018-5291
eISSN
2074-0131
DOI
10.1177/10185291221117197
Publisher site
See Article on Publisher Site

Abstract

In this study, 13 bacteria were isolated from the agricultural soils of Kyaukse city, Myanmar, and out of these isolates, two bacteria isolates viz. JU-24 and JU-33 are found to be potent phosphate (P) solubilisers and are selected for further studies. These two isolates are identified as Bacillus megaterium based on the 16S rRNA gene sequence. When the growth rates of these two isolates were tested for salt tolerance, they could grow well in media with NaCl concentrations of 3%, 6% and 9%. For 0% and 3% NaCl concentrations, JU-33 (84.37 mg/L for 0% NaCl and 130.36 mg/L for 3% NaCl) showed higher P-solubilising efficiency than JU-24 (73.29 mg/L for 0% NaCl and 87.42 mg/L for 3% NaCl) for both NaCl concentrations when inoculated in Ca3(PO4)2 containing liquid medium. In media containing AlPO4 and FePO4 without NaCl, the P-solubilising activity of JU-24 is higher than JU-33 for these two substrates. Moreover, these strains showed noticeable levels of P solubilisation activity in the presence of various carbon sources indicating high P-solubilising efficacy. The soil inoculation experiment revealed that inoculating the experimental soil with JU-33 resulted in an obvious increase in available P, which increased from 13.08 mg/L in the non-inoculated soil to 18.40 mg/L in the inoculated soil with the bacterial isolate, JU-33. This study clearly showed that JU-24 and JU-33 isolates could be used as biofertilizers in ecological agricultural systems and may help to sustain environmental health and soil productivity. The use of biofertilizers can reduce the use of expensive chemical fertilisers, reducing planting costs and improving soil fertility through long-term use. Biofertilizers can help solve the problem of feeding an increasing global population at a time when agriculture is facing various environmental stresses and are affordable for low-income farmers.

Journal

Asia-Pacific Journal of Rural Development – SAGE

Published: Jun 1, 2022

Keywords: Bacillus megaterium; carbon sources; phosphate solubilising bacteria; salt-tolerant; P substrates

References

  • Microbial mobilization of soil phosphorus and sustainable P management in agricultural soils
    Adhya, T. K.; Kumar, N.; Reddy, G.; Podile, A. R.; Bee, H.; Samantaray, B.
  • Health sector reform and human resources: Lessons from the United Kingdom
    Buchan, J.
  • Halotolerant aerobic heterotrophic bacteria from the Great Salt Plains of Oklahoma
    Caton, T. M.; Witte, L. R.; Ngyuen, H. D.; Buchheim, J. A.; Buchheim, M. A.; Schneegurt, M. A.
  • Screening and identification of phosphate-solubilizing bacteria from reclaimed soil in Shanxi mining area
    Chen, Q.; Liu, S.; Bai, Y.; Li, Y.; Gao, C.; Zhang, Q.
  • [Diversity of halophilic and halotolerant bacteria isolated from non-saline soil collected from Xiaoxi National Natural Reserve, Hunan Province]
    Chen, Q.; Liu, Z.; Peng, Q.; Huang, K.; He, J.; Zhang, L.; Li, W.; Chen, Y.
  • Availability of organic and inorganic phosphorus compounds as phosphorus sources for Carex species
    Corona, M. E. P.; Pérez Corona, M. E.; Van Der Klundert, I.; Verhoeven, J. T. A.
  • Identification of rhizosphere bacterial diversity with promising salt tolerance, PGP traits and their exploitation for seed germination enhancement in sodic soil
    Damodaran, T.; Mishra, V. K.; Jha, S. K.; Pankaj, U.; Gupta, G.; Gopal, R.
  • Biogeochemical processes in the continental slope of Bay of Bengal: I. Bacterial solubilization of inorganic phosphate
    Das, S.; Lyla, P. S.; Khan, S. A.
  • Endospores of halophilic bacteria of the family Bacillaceae isolated from non-saline Japanese soil may be transported by Kosa event (Asian dust storm)
    Echigo, A.; Hino, M.; Fukushima, T.; Mizuki, T.; Kamekura, M.; Usami, R.
  • Processes governing phosphorus availability in temperate soils
    Frossard, E.; Condron, L. M.; Oberson, A.; Sinaj, S.; Fardeau, J. C.
  • Isolation and identification of phosphate solubilizing bacteria able to enhance the growth and aloin-A biosynthesis of Aloe barbadensis Miller
    Gupta, M.; Kiran, S.; Gulati, A.; Singh, B.; Tewari, R.
  • Role of halotolerant phosphate-solubilising bacteria on growth promotion of peanut (Arachis hypogaea) under saline soil
    Jiang, H.; Qi, P.; Wang, T.; Chi, X.; Wang, M.; Chen, M.; Chen, N.; Pan, L.
  • Alleviation of salt stress by enterobacter sp. EJ01 in tomato and Arabidopsis is accompanied by up-regulation of conserved salinity responsive factors in plants
    Kim, K.; Jang, Y.-J.; Lee, S.-M.; Oh, B.-T.; Chae, J.-C.; Lee, K.-J.
  • A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences
    Kimura, M.
  • Interactions between Laccaria laccata, Agrobacterium radiobacter and beech roots: Influence on P, K, Mg, and Fe mobilization from minerals and plant growth
    Leyval, C.; Berthelin, J.
  • A study of soil bacteria dissolving certain mineral phosphate fertilizers and related compounds
    Louw, H. A.; Webley, D. M.
  • Utilization of molecular markers for improving the phosphorus efficiency in crop plants
    Maharajan, T.; Ceasar, S. A.; Krishna, T. P.; Ramakrishnan, M.; Duraipandiyan, V.; Naif-Abdulla, A.-D.; Ignacimuthu, S.
  • Mechanisms of salinity tolerance
    Munns, R.; Tester, M.
  • Microbial ACC-deaminase: Prospects and applications for inducing salt tolerance in plants
    Nadeem, S. M.; Zahir, Z. A.; Naveed, M.; Ashraf, M.
  • Stress induced phosphate solubilization in bacteria isolated from alkaline soils
    Nautiyal, C. S.; Bhadauria, S.; Kumar, P.; Lal, H.; Mondal, R.; Verma, D.
  • Phenotypic and genotypic characterization of phosphate solubilizing bacteria and their efficiency on the growth of maize
    Pande, A.; Pandey, P.; Mehra, S.; Singh, M.; Kaushik, S.
  • Isolation and characterization of salt tolerant phosphate solubilizing Serratia marcescens isolated from coastal area
    Patel, K.; Patel, S.; Parekh, V.; Jha, S.
  • Phosphate solubilization potential and phosphatase activity of some bacterial strains isolated from thermal power plant effluent exposed water of river Ganga
    Paul, D.; Sinha, S. N.
  • Effect of phosphate-solubilizing bacteria and vesicular-arbuscular mycorrhizal fungi on the utilization of Bayovar rock phosphate by alfalfa plants using a sand-vermiculite medium
    Piccini, D.; Azcon, R.
  • Mobilization of phosphorus in soil connection with the vital activity of some microbial species
    Pikovskaya, R. I.
  • Phosphate solubilization mechanisms in alkaliphilic bacterium Bacillus marisflavi FA7
    Prabhu, N.; Borkar, S.; Garg, S.
  • Economics of salt-induced land degradation and restoration
    Qadir, M.; Quillérou, E.; Nangia, V.; Murtaza, G.; Singh, M.; Thomas, R. J.; Drechsel, P.; Noble, A. D.
  • Osmoadaptation and plant growth promotion by salt tolerant bacteria under salt stress
    Qurashi, A. W.; Sabri, A. N.
  • Role of different carbon sources in phosphate solubilization
    Rathore, P.
  • Phosphate solubilizing bacteria and their role in plant growth promotion
    Rodríguez, H.; Fraga, R.
  • The neighbor-joining method: A new method for reconstructing phylogenetic trees
    Saitou, N.; Nei, M.
  • Phosphorous and phosphate solubilising bacteria and their role in plant nutrition
    Satyaprakash, M.; Nikitha, T.; Reddi, E. U. B.; Sadhana, B.; Vani, S. S.
  • Improving salinity tolerance in cereals
    Shahbaz, M.; Ashraf, M.
  • Phosphate solubilizing bacteria-assisted salinity tolerance in plants: A review
    Sharma, P.; Baishya, R.
  • Maximizing root/rhizosphere efficiency to improve crop productivity and nutrient use efficiency in intensive agriculture of China
    Shen, J.; Li, C.; Mi, G.; Li, L.; Yuan, L.; Jiang, R.; Zhang, F.
  • Alleviation of salinity-induced damage on wheat plant by an ACC deaminase-producing halophilic bacterium Serratia sp. SL-12 isolated from a salt lake
    Singh, R. P.; Jha, P. N.
  • Phosphate solubilization by soil microorganisms
    Subba Rao, N. S.
  • C. A. B. International & British Society of Soil Science
    Syers, J. K.; Rimmer, D. L.
  • Soil salinity, household wealth and food insecurity in tropical deltas: Evidence from south-west coast of Bangladesh
    Szabo, S.; Hossain, M. S.; Adger, W. N.; Matthews, Z.; Ahmed, S.; Lázár, A. N.; Ahmad, S.
  • MEGA6: Molecular evolutionary genetics analysis version 6.0
    Tamura, K.; Stecher, G.; Peterson, D.; Filipski, A.; Kumar, S.
  • The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools
    Thompson, J. D.; Gibson, T. J.; Plewniak, F.; Jeanmougin, F.; Higgins, D. G.
  • Diversity of Paenibacillus polymyxa strains isolated from the rhizosphere of maize planted in Cerrado soil
    von der Weid, I.; Paiva, E.; Nóbrega, A.; van Elsas, J. D.; Seldin, L.
  • Screening of P solubilizing bacteria and its effects on transformation of soil inorganic P fractions
    Yu, Q.; Ma, Z.; Wang, J.; Wei, L.
  • Plants growth promoting rhizobacteria: Applications and perspectives in agriculture
    Zahir, Z. A.; Arshad, M.; Frankenberger, W. T.