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Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas aeruginosa Strains

Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas... Original Article Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas aeruginosa Strains 1 1 1 1 1 Fatemeh Alimoradi , Hosein Eslami , Ali Taghavi Zeneuz , Tahmoores Abdollahian , Arash Jangjoo , 2,3 2,3 Parisa Raei , Hossein Samadi Kafil Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran, Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ABSTRA CT Aims and Objectives: Pseudomonas aeruginosa is one of the bacteria which exist in oral cavity sores and is challenging for oral diseases specialists. The development of resistance to antibiotics has caused researchers to think of using new treatment approaches such as phage therapy. This study aims to identify and isolate the bacteriophage that is effective on P. aeruginosa causing oral infections. The present research is a laboratory study on six Pseudomonas spp. strains with different carbapenemase-resistant genes. Materials and Methods: Specific phages were isolated from enriched sludge (microbial pentavalent compound). After isolation, to examine the physical and biological structure of bacteriophage, uracil negative staining protocol was used, and finally, the structure was observed using http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Journal of Advanced Oral Research SAGE

Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas aeruginosa Strains

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Publisher
SAGE
Copyright
© 2016 Academy of Advanced Dental Research
ISSN
2320-2068
eISSN
2320-2076
DOI
10.1177/2229411220160207
Publisher site
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Abstract

Original Article Isolation and Identification of Bacteriophage Effective on Carbapenemase-resistant Pseudomonas aeruginosa Strains 1 1 1 1 1 Fatemeh Alimoradi , Hosein Eslami , Ali Taghavi Zeneuz , Tahmoores Abdollahian , Arash Jangjoo , 2,3 2,3 Parisa Raei , Hossein Samadi Kafil Department of Oral and Maxillofacial Medicine, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran, Infectious Disease and Tropical Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran, Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran ABSTRA CT Aims and Objectives: Pseudomonas aeruginosa is one of the bacteria which exist in oral cavity sores and is challenging for oral diseases specialists. The development of resistance to antibiotics has caused researchers to think of using new treatment approaches such as phage therapy. This study aims to identify and isolate the bacteriophage that is effective on P. aeruginosa causing oral infections. The present research is a laboratory study on six Pseudomonas spp. strains with different carbapenemase-resistant genes. Materials and Methods: Specific phages were isolated from enriched sludge (microbial pentavalent compound). After isolation, to examine the physical and biological structure of bacteriophage, uracil negative staining protocol was used, and finally, the structure was observed using

Journal

Journal of Advanced Oral Research – SAGE

Published: May 1, 2016

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