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Downregulation of expression of transporters associated with antigen processing 1 and 2 and human leukocyte antigen I and its effect on immunity in nasopharyngeal carcinoma patients

Downregulation of expression of transporters associated with antigen processing 1 and 2 and human... The human leukocyte antigen (HLA)‑I and antigen‑processing machinery (APM) are crucial in the anticancer immune response. The aim of this study was to assess the clinical significance of the APM components (transporters associated with antigen processing (TAP)-1 and ‑2 and HLA‑I) in nasopharyngeal carcinoma (NPC). A total of 58 NPC specimens and 20 healthy specimens used as control were evaluated by semiquantitative immunohistochemistry for three APM components (TAP‑1, TAP‑2 and HLA‑I). The expression of the APM components in NPC was downregulated. CD4+ and CD8+ T cells were measured by flow cytometry and IL‑10 was measured by ELISA. The number of CD8+ T cells and the expression of IL‑10 were higher and the number of CD4+ T cells was lower in NPC, compared to the controls. The number of CD8+ T cells and the expression of IL‑10 were negatively correlated with TAP‑1, TAP‑2 and HLA‑I expression. The clinical phase, lymph node metastasis, distant metastasis, pathological type, TAP‑1 expression, TAP‑2 expression and HLA‑I expression were identified as prognostic factors by the Kaplan‑Meier analysis. A multivariate analysis using a Cox regression model indicated that distant metastasis and the downregulation of HLA‑Ⅰ expression were independent unfavorable prognostic factors. In conclusion, the lower expression of HLA‑I induced immunosuppression in NPC patients and was associated with a poor prognosis. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular and Clinical Oncology Spandidos Publications

Downregulation of expression of transporters associated with antigen processing 1 and 2 and human leukocyte antigen I and its effect on immunity in nasopharyngeal carcinoma patients

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References (29)

Publisher
Spandidos Publications
Copyright
Copyright © Spandidos Publications
ISSN
2049-9450
eISSN
2049-9469
DOI
10.3892/mco.2013.194
Publisher site
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Abstract

The human leukocyte antigen (HLA)‑I and antigen‑processing machinery (APM) are crucial in the anticancer immune response. The aim of this study was to assess the clinical significance of the APM components (transporters associated with antigen processing (TAP)-1 and ‑2 and HLA‑I) in nasopharyngeal carcinoma (NPC). A total of 58 NPC specimens and 20 healthy specimens used as control were evaluated by semiquantitative immunohistochemistry for three APM components (TAP‑1, TAP‑2 and HLA‑I). The expression of the APM components in NPC was downregulated. CD4+ and CD8+ T cells were measured by flow cytometry and IL‑10 was measured by ELISA. The number of CD8+ T cells and the expression of IL‑10 were higher and the number of CD4+ T cells was lower in NPC, compared to the controls. The number of CD8+ T cells and the expression of IL‑10 were negatively correlated with TAP‑1, TAP‑2 and HLA‑I expression. The clinical phase, lymph node metastasis, distant metastasis, pathological type, TAP‑1 expression, TAP‑2 expression and HLA‑I expression were identified as prognostic factors by the Kaplan‑Meier analysis. A multivariate analysis using a Cox regression model indicated that distant metastasis and the downregulation of HLA‑Ⅰ expression were independent unfavorable prognostic factors. In conclusion, the lower expression of HLA‑I induced immunosuppression in NPC patients and was associated with a poor prognosis.

Journal

Molecular and Clinical Oncology – Spandidos Publications

Published: Jan 1, 2014

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