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A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments

A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Molecular Biotechnology Springer Journals

A Simple Silica-based Method for Metagenomic DNA Extraction from Soil and Sediments

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 2008 by Humana Press
Subject
Chemistry; Human Genetics ; Biological Techniques; Protein Science; Cell Biology; Biochemistry, general; Biotechnology
ISSN
1073-6085
eISSN
1559-0305
DOI
10.1007/s12033-008-9061-8
pmid
18373226
Publisher site
See Article on Publisher Site

Abstract

A new method is described for extraction of metagenomic DNA from soil and sediments which is based on DNA adsorption to silica without the use of phenol, ethanol precipitation or a cesium chloride gradient. High-quality DNA was obtained, and PCR inhibition was overcome by adding bovine serum albumin and adjusting magnesium concentration. By using PCR-DGGE with Firmicutes and lactic acid bacteria-specific primers the extracted metagenomic DNA was shown to contain a mixture of bacterial genomes. This method can be used for screening bacterial diversity in soil and sediment samples.

Journal

Molecular BiotechnologySpringer Journals

Published: Mar 29, 2008

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