Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

A Toxicologist's Guide to Clinical Pathology in AnimalsBlood and Urine Sampling

A Toxicologist's Guide to Clinical Pathology in Animals: Blood and Urine Sampling [Every clinical pathology measurement can be undermined by poor sample collection, and there are many ways that samples can be compromised. A long list of tests can be ruined by red blood cell hemolysis and the presence of bilirubin because of improper blood collection technique. Similarly, a failure to properly fast animals can cause serum to be lipemic, which can interfere with many tests. Blood should be drawn from the same site, and at the same time of day throughout serial samplings. This is essential in rodents since their leukocyte counts fluctuate widely due to circadian effects and sampling site. Cell counts in all species differ depending on the collection site. When an animal is stressed during blood collection, there is a release of corticosteroids, splenic contraction, and the release of additional cells into circulation; and blood chemistries are also affected. Four methods for collecting urine are described along with their strengths and weaknesses. There is no ideal way to preserve urine so the means used are dictated by the evaluations needed.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

A Toxicologist's Guide to Clinical Pathology in AnimalsBlood and Urine Sampling

Whalan, John E.
Springer Journals — Jan 31, 2015
Download PDF
6 pages

Loading next page...
 
/lp/springer-journals/a-toxicologist-s-guide-to-clinical-pathology-in-animals-blood-and-S3W0eHyZ2O
Publisher
Springer International Publishing
Copyright
© Springer International Publishing Switzerland 2015
ISBN
978-3-319-15852-5
Pages
27 –33
DOI
10.1007/978-3-319-15853-2_3
Publisher site
See Chapter on Publisher Site

Abstract

[Every clinical pathology measurement can be undermined by poor sample collection, and there are many ways that samples can be compromised. A long list of tests can be ruined by red blood cell hemolysis and the presence of bilirubin because of improper blood collection technique. Similarly, a failure to properly fast animals can cause serum to be lipemic, which can interfere with many tests. Blood should be drawn from the same site, and at the same time of day throughout serial samplings. This is essential in rodents since their leukocyte counts fluctuate widely due to circadian effects and sampling site. Cell counts in all species differ depending on the collection site. When an animal is stressed during blood collection, there is a release of corticosteroids, splenic contraction, and the release of additional cells into circulation; and blood chemistries are also affected. Four methods for collecting urine are described along with their strengths and weaknesses. There is no ideal way to preserve urine so the means used are dictated by the evaluations needed.]

Published: Jan 31, 2015

Keywords: Calcium Oxalate; Sodium Oxalate; Metaphosphoric Acid; Calcium Citrate; Potassium Oxalate

There are no references for this article.