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Adenosine A1 receptor-mediated transactivation of the EGF receptor produces a neuroprotective effect on cortical neurons in vitro

Adenosine A1 receptor-mediated transactivation of the EGF receptor produces a neuroprotective... Acta Pharmacologica Sinica (2009) 30: 889–898 npg © 2009 CPS and SIMM All rights reserved 1671-4083/09 $32.00 www.nature.com/aps Original Article Adenosine A receptor-mediated transactivation of the EGF receptor produces a neuroprotective effect on cortical neurons in vitro 1,# 1,# 1 2 1, Ke-qiang XIE , Li-min ZHANG , Yan CAO , Jun ZHU , Lin-yin FENG State Key Laboratory of Drug Research, Department of Neuropharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China Aim: To understand the mechanism of the transactivation of the epidermal growth factor receptor (EGFR) mediated by the adenosine A receptor (A R). 1 1 Methods: Primary cultured rat cortical neurons subjected to oxygen-glucose deprivation (OGD) and HEK293/A R cells were treated with the A R-specific agonist N -cyclopentyladenosine (CPA). Phospho-EGFR, Akt, and ERK1/2 were observed by Western blot. An interac- tion between EGFR and A R was detected using immunoprecipitation and immunocytochemistry. Results: The A R agonist CPA causes protein kinase B (Akt) activation and protects primary cortical neurons from oxygen-glucose depri- vation (OGD) insult. A R and EGFR co-localize in the membranes of neurons and form an immunocomplex. A R http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Acta Pharmacologica Sinica Springer Journals

Adenosine A1 receptor-mediated transactivation of the EGF receptor produces a neuroprotective effect on cortical neurons in vitro

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References (37)

Publisher
Springer Journals
Copyright
Copyright © 2009 by CPS and SIMM
Subject
Biomedicine; Biomedicine, general; Pharmacology/Toxicology; Medical Microbiology; Immunology; Internal Medicine; Vaccine
ISSN
1671-4083
eISSN
1745-7254
DOI
10.1038/aps.2009.80
Publisher site
See Article on Publisher Site

Abstract

Acta Pharmacologica Sinica (2009) 30: 889–898 npg © 2009 CPS and SIMM All rights reserved 1671-4083/09 $32.00 www.nature.com/aps Original Article Adenosine A receptor-mediated transactivation of the EGF receptor produces a neuroprotective effect on cortical neurons in vitro 1,# 1,# 1 2 1, Ke-qiang XIE , Li-min ZHANG , Yan CAO , Jun ZHU , Lin-yin FENG State Key Laboratory of Drug Research, Department of Neuropharmacology, Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai 201203, China; Department of Neurology, Huashan Hospital, Fudan University, Shanghai 200040, China Aim: To understand the mechanism of the transactivation of the epidermal growth factor receptor (EGFR) mediated by the adenosine A receptor (A R). 1 1 Methods: Primary cultured rat cortical neurons subjected to oxygen-glucose deprivation (OGD) and HEK293/A R cells were treated with the A R-specific agonist N -cyclopentyladenosine (CPA). Phospho-EGFR, Akt, and ERK1/2 were observed by Western blot. An interac- tion between EGFR and A R was detected using immunoprecipitation and immunocytochemistry. Results: The A R agonist CPA causes protein kinase B (Akt) activation and protects primary cortical neurons from oxygen-glucose depri- vation (OGD) insult. A R and EGFR co-localize in the membranes of neurons and form an immunocomplex. A R

Journal

Acta Pharmacologica SinicaSpringer Journals

Published: Jul 3, 2009

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