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Advances in Physarum MachinesLong-Term Storable Microfluidic Whole-Cell Biosensor Using Physarum polycephalum for Toxicity Prescreening

Advances in Physarum Machines: Long-Term Storable Microfluidic Whole-Cell Biosensor Using... [A storable whole-cell biosensor has been a challenge in the whole-cell biosensor research. We developed a long-term storable whole-cell biosensor using the true slime mould, Physarum polycephalum, for toxicity detection. The cell is interfaced to a microfluidic device with impedance measurement system. The oscillatory activity of the cell when exposed to various concentrations of 2,4-dinitrophenol (DNP) is investigated. It has been demonstrated that the Physarum cell can be dry-stored in the device for months and used as bionsensor after revived with rehydration. This is the first implementation of storable whole-cell biosensor for toxicity detection use, and it suggests that the development of long-term storable, and potentially portable, whole-cell biosensor for general toxicity prescreening is possible.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

Advances in Physarum MachinesLong-Term Storable Microfluidic Whole-Cell Biosensor Using Physarum polycephalum for Toxicity Prescreening

Part of the Emergence, Complexity and Computation Book Series (volume 21)
Editors: Adamatzky, Andrew
Tsuda, Soicdhiro; Zauner, Klaus-Peter; Morgan, Hywel
Advances in Physarum Machines — Jan 10, 2016
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11 pages

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Publisher
Springer International Publishing
Copyright
© Springer International Publishing Switzerland 2016
ISBN
978-3-319-26661-9
Pages
153 –164
DOI
10.1007/978-3-319-26662-6_8
Publisher site
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Abstract

[A storable whole-cell biosensor has been a challenge in the whole-cell biosensor research. We developed a long-term storable whole-cell biosensor using the true slime mould, Physarum polycephalum, for toxicity detection. The cell is interfaced to a microfluidic device with impedance measurement system. The oscillatory activity of the cell when exposed to various concentrations of 2,4-dinitrophenol (DNP) is investigated. It has been demonstrated that the Physarum cell can be dry-stored in the device for months and used as bionsensor after revived with rehydration. This is the first implementation of storable whole-cell biosensor for toxicity detection use, and it suggests that the development of long-term storable, and potentially portable, whole-cell biosensor for general toxicity prescreening is possible.]

Published: Jan 10, 2016

Keywords: Impedance Measurement; Physarum Polycephalum; Ferric Chloride Solution; Protoplasmic Streaming; Film Photoresist

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