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Bacterial symbionts associated with an octopus were isolated and their intra and extracellular extracts were tested for the antagonistic activities against biofilm-forming bacteria. Among the three antagonistic bacterial symbionts, both intra and extracellular extracts of the bacterium, Pseudomonas putida exhibited strong antibacterial activity against biofilm bacteria. Results also showed that both intra and extracellular extracts of P. putida significantly reduced the biofilm formation on test surfaces. The antifouling coating developed with bacterial extracts of P. putida significantly reduced the recruitment of biofouling organisms over a period of 50 days in the coastal waters. The bioactive fractions were partially purified using chromatographic methods such as thin-layer chromatography and high performance liquid chromatography. Based on the findings, it is concluded that potential antifouling compounds could be isolated from the antagonistic bacteria isolated from the octopus.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: Dec 17, 2015
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