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Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus

Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an... Bacterial symbionts associated with an octopus were isolated and their intra and extracellular extracts were tested for the antagonistic activities against biofilm-forming bacteria. Among the three antagonistic bacterial symbionts, both intra and extracellular extracts of the bacterium, Pseudomonas putida exhibited strong antibacterial activity against biofilm bacteria. Results also showed that both intra and extracellular extracts of P. putida significantly reduced the biofilm formation on test surfaces. The antifouling coating developed with bacterial extracts of P. putida significantly reduced the recruitment of biofouling organisms over a period of 50 days in the coastal waters. The bioactive fractions were partially purified using chromatographic methods such as thin-layer chromatography and high performance liquid chromatography. Based on the findings, it is concluded that potential antifouling compounds could be isolated from the antagonistic bacteria isolated from the octopus. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Antifouling Activities of Antagonistic Marine Bacterium Pseudomonas putida Associated with an Octopus

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References (56)

Publisher
Springer Journals
Copyright
Copyright © 2015 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-015-0691-x
Publisher site
See Article on Publisher Site

Abstract

Bacterial symbionts associated with an octopus were isolated and their intra and extracellular extracts were tested for the antagonistic activities against biofilm-forming bacteria. Among the three antagonistic bacterial symbionts, both intra and extracellular extracts of the bacterium, Pseudomonas putida exhibited strong antibacterial activity against biofilm bacteria. Results also showed that both intra and extracellular extracts of P. putida significantly reduced the biofilm formation on test surfaces. The antifouling coating developed with bacterial extracts of P. putida significantly reduced the recruitment of biofouling organisms over a period of 50 days in the coastal waters. The bioactive fractions were partially purified using chromatographic methods such as thin-layer chromatography and high performance liquid chromatography. Based on the findings, it is concluded that potential antifouling compounds could be isolated from the antagonistic bacteria isolated from the octopus.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: Dec 17, 2015

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