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References (29)
-
Z Yang, Y Ding, Y Zhang, F Liu (2008)
Rapid purification of truncated Taq DNA polymerase Stoffel fragments by boiling lysis of bacterial expression culturesBiotechnol Appl Biochem, 50
-
RA Harell, RP Hart (1994)
Rapid preparation of Thermus flavus DNA polymeraseGenome Res, 3
-
N Kurosawa, YH Itoh, T Itoh (2005)
Thermus kawarayensis sp. nov., a new member of the genus Thermus, isolated from Japanese hot springsExtremophiles, 9
-
SF Altschul, LM Thomas, AS Alejandro, Z Jinghui, Z Zhang, M Webb, JL David (1997)
Gaped BLAST and PSIBLAST: a new generation of protein database search programNucleic Acid Res, 25
-
MC Srinivasan (1994)
Microbial biodiversity and its relevance to screening for novel industrially useful enzymesCurr Sci, 66
-
M Roayaei, H Galehdari (2008)
Cloning and expression of Thermus aquaticus DNA polymerase in Escherichia coliJundishapur J Microbiol, 1
-
TD Brock, H Freeze (1969)
Thermus aquaticus gen. n and sp. n. a nonsproulating extreme thermophileJ Bacteriol, 98
-
M Sharma, NN Sharma, TC Bhalla (2012)
Purification studies on a thermo-active amidase of Geobacillus pallidus BTP-5x MTCC 9225 isolated from thermal springs of Tatapani, Himachal PradeshAppl Biochem Biotechnol, 10
-
RF Ramaley, Hixson (1970)
Isolation of a non-pigmented, thermophilic bacterium similar to Thermus aquaticusJ Bacteriol, 103
-
RK Scopes (1982)
Protein purification: principal and practice -
S Trivedi, HS Gehlot, SR Rao (2006)
Protein thermostability in Archaea and EubacteriaGenet Mol Res, 5
-
P Saha, AK Mondal, S Mayilraj, S Krishnamurthi, A Bhattacharya, S Chakrabarti (2005)
Paenibacillus assamensis sp nov, a novel bacterium isolated from a warm spring in Assam, IndiaInt J Syst Evol Microbiol, 55
-
P Ferrali, JD Egan, LE Floyd (2007)
Making Taq DNA polymerase in the undergraduate biology laboratoryBios, 78
-
S Sato, JI Harris (1977)
Isolated and characterized superoxide dismutase from the extreme thermophile Thermus aquaticusEur J Biochem, 73
-
T Imperio, C Viti, L Marri (2008)
Alicyclobacillus pohliae sp. nov., a thermophilic, endospore forming bacterium isolated from geothermal soil of the north–west slope of Mount Melbourne (Antarctica)Int J Syst Evol Microbiol, 58
-
JK Kristjansson, Hreggvidson, GA Alfredsson (1986)
Isolation of halotolerant Thermus spp. from submarine hot springs in IcelandAppl Environ Microbiol, 52
-
K Mullis, F Faloona (1986)
Specific enzymatic amplification of DNA in vitro: the polymerase chain reactionCold Spring Harb Symp Quant Biol, 51
-
M Obeidat, HK Horani, A Zoubi, I Otri (2012)
Isolation, characterization, and hydrolytic activities of Geobacillus species from Jordanian hot springsAfr J Biotechnol, 11
-
J Cline, J Braman, H Hogrefe (1996)
PCR fidelity of Pfu DNA polymerase and other DNA polymerasesNucleic Acids Res, 24
-
TD Brock (1967)
Life at high temperaturesScience, 158
-
SS Bisht, AK Panda (2011)
Biochemical characterization and 16S rRNA sequencing of few lipase producing thermophilic bacteria from Taptapani hot water spring, Orissa, IndiaBiotechnol Res Int, 11
-
A Khalil (2011)
Screening and characterization of thermophilic bacteria (lipase, cellulose and amylase producers) from hot springs in Saudi ArabiaJ Food Agric Environ, 9
-
P Hugenholtz, C Pitulle, KL Hershbergerand, NR Pace (1998)
Novel division level bacterial diversity in a Yellowstone hot springJ Bacteriol, 180
-
A Chien, DB Edgar, JM Trela (1976)
Deoxyribonucleic acid polymerase from the extreme thermophile, Thermus aquaticusJ Bacteriol, 127
-
FC Lawyer, S Stoffel, RK Saiki (1993)
High-level expression, purification and enzymatic characterization of full-length Thermus aquaticus DNA polymerasePCR Methods Appl, 2
-
RW Castenholz (1969)
Thermophilic blue-green algae and the thermal environmentBacteriol Rev, 33
-
S Acharya, A Chaudhary (2012)
Alkaline cellulase produced by a newly isolated thermophilic Aneurinibacillus thermoaerophilus WBS2 from hot spring, IndiaAfr J Microbiol Res, 6
-
KR Aneja (2003)
Experiments in microbiology, plant pathology and biotechnology -
C Guo, T Wang, W Zhu, D Zhang, X Cui, L Xu, Q Peng (2003)
The phylotype of Thermus from Rehai geothermal area, Tengchong, ChinaJ Microbiol, 41
- Publisher
- Springer Journals
- Copyright
- Copyright © 2014 by The National Academy of Sciences, India
- Subject
- Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
- ISSN
- 0369-8211
- eISSN
- 2250-1746
- DOI
- 10.1007/s40011-014-0412-x
- Publisher site
- See Article on Publisher Site
Abstract
Thermophilic microorganisms are adapted to live at high temperatures and produce many thermostable enzymes such as Taq polymerase, amylase, lipase, protease, etc., which find a number of commercial applications because of their thermostability. Thermal springs of Manikaran, Vashisht, Khirganga, Tattapani and Jeory of districts Kullu, Mandi and Shimla respectively, were selected for the present study and forty two thermophilic bacterial isolates were isolated using Castenholz trypticase yeast extract medium. All these thermophilic bacterial isolates were screened using morphological and biochemical characters. For molecular characterization of the selected isolates, Polymerase chain reaction was carried out for the amplification of the DNA using genus specific primers for 16S ribosomal RNA gene. Nucleotide sequences obtained after sequencing were blasted and only two isolates were found to belong to genus Thermus and the strain MS3 was found to possess maximum homology of 99 % with Thermus aquaticus. The growth parameters optimized were found to be pH of 7.5, incubation temperature 65 °C and incubation time 96 h. Taq polymerase produced using selected strain MS3, was purified by ammonium sulphate precipitation, gel permeation chromatography and ion exchange chromatography. The Taq polymerase assay with 1.5 µl purified enzyme fraction obtained after ion exchange chromatography, yielded comparable results with 0.5 µl (3.0 U/µl) of commercial Taq DNA polymerase, indicating that the protein fraction has 1.0 U/µl of DNA polymerase activity. Molecular mass of the purified Taq DNA polymerase was found to be 94,000 daltons. Protein sequencing analysis of the purified enzyme product showed 99 % homology with A-Chain structure of Taq DNA polymerase with accession number 1TAQ A.
Journal
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: Sep 6, 2014