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Characterization and Strain Improvement of Aerobic Denitrifying EPS Producing Bacterium Bacillus cereus PB88 for Shrimp Water Quality Management

Characterization and Strain Improvement of Aerobic Denitrifying EPS Producing Bacterium Bacillus... A bacterial strain PB88 was isolated from intensive shrimp culture pond to study the denitrification process. On the basis of 16S rDNA analysis, strain PB88 was identified as Bacillus cereus PB88. It has the potential to remove 82.33 ± 3.24% $${\text{NO}}_{2}^{ - } {-} {\text{N}}$$ NO 2 - - N in synthetic medium. The optimum pH, temperature and dissolved oxygen for the highest denitrification process of the PB88 were 8.0, 30 °C and 5.21 mg/l (150 rpm) respectively. PB88 harbour the genetic sequence of nitrite reductase (nirS) enzyme which is essential to complete aerobic denitrification process. One remarkable finding is that the experimental organism produced exopolysaccharide (EPS) during the denitrification process and EPS has the antibacterial property against shrimp pathogen Vibrio harveyi MTCC 7954 (inhibition zone of 5.21 mm) and Vibrio vulnificus MTCC 1145 (inhibition zone of 7.11 mm). Removal of $${\text{NO}}_{2}^{ - } {-} {\text{N}}$$ NO 2 - - N in open base shrimp wastewater system were recorded as 98.51% by B. cereus PB88 and average shrimp body weight gained in treated system as 6 ± 0.54 to 8 ± 0.74 g within 7 days. Overall result indicated that B. cereus PB88 has the immense potential for the application in commercial shrimp culture as denitrifying agent. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Waste and Biomass Valorization Springer Journals

Characterization and Strain Improvement of Aerobic Denitrifying EPS Producing Bacterium Bacillus cereus PB88 for Shrimp Water Quality Management

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References (47)

Publisher
Springer Journals
Copyright
Copyright © 2017 by Springer Science+Business Media Dordrecht
Subject
Engineering; Engineering, general; Environment, general; Renewable and Green Energy; Industrial Pollution Prevention; Waste Management/Waste Technology; Environmental Engineering/Biotechnology
ISSN
1877-2641
eISSN
1877-265X
DOI
10.1007/s12649-017-9912-2
Publisher site
See Article on Publisher Site

Abstract

A bacterial strain PB88 was isolated from intensive shrimp culture pond to study the denitrification process. On the basis of 16S rDNA analysis, strain PB88 was identified as Bacillus cereus PB88. It has the potential to remove 82.33 ± 3.24% $${\text{NO}}_{2}^{ - } {-} {\text{N}}$$ NO 2 - - N in synthetic medium. The optimum pH, temperature and dissolved oxygen for the highest denitrification process of the PB88 were 8.0, 30 °C and 5.21 mg/l (150 rpm) respectively. PB88 harbour the genetic sequence of nitrite reductase (nirS) enzyme which is essential to complete aerobic denitrification process. One remarkable finding is that the experimental organism produced exopolysaccharide (EPS) during the denitrification process and EPS has the antibacterial property against shrimp pathogen Vibrio harveyi MTCC 7954 (inhibition zone of 5.21 mm) and Vibrio vulnificus MTCC 1145 (inhibition zone of 7.11 mm). Removal of $${\text{NO}}_{2}^{ - } {-} {\text{N}}$$ NO 2 - - N in open base shrimp wastewater system were recorded as 98.51% by B. cereus PB88 and average shrimp body weight gained in treated system as 6 ± 0.54 to 8 ± 0.74 g within 7 days. Overall result indicated that B. cereus PB88 has the immense potential for the application in commercial shrimp culture as denitrifying agent.

Journal

Waste and Biomass ValorizationSpringer Journals

Published: Mar 28, 2017

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