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Characterization of a Bacterial Culture Collection from Terrestrial Subsurface Habitats in Colombia

Characterization of a Bacterial Culture Collection from Terrestrial Subsurface Habitats in Colombia Microorganisms in terrestrial subsurface habitats have important roles in rock and mineral transformation and biogeochemical cycles processes. A group of 194 bacteria strains from three Colombian terrestrial subsurface habitats [oil fields (OF), coal mines (CM) and mud volcanoes (MV)] were characterized using both phylogenetic and phenotypic analyses. Hill´s number and related diversity indices were computed to know collection diversity and its relationship with sampling sites. Phylogenetic tree based on 16S rRNA gene dissimilarity values between strains showed seven major taxonomic/phylogenetic groups (α-, β-, δ-, γ-Proteobacteria, Actinobacteria, Clostridia and Bacilli) in culture collection, which contain sixty-one (61) putative bacteria species grouped in twenty-eight (28) genera. The widest represented genera of the culture collection were Bacillus, Beijerinckia, Cutibacterium, Desulfovibrio, Halomonas, Marinobacter, Pseudomonas, and Zobellella. The genus richness (q0) varied through sampling sites (MV > CM > OF). By the contrary, genus diversity based on relative abundances (q1) or relative abundances-evenness (q2) showed the follows diversity profiles: q1—(MV > OF > CM); q2—(MV > OF > CM). Genus’s abundances and evenness in overall collection resulted between moderate and high. The culture collection also showed bacterial groups with diverse metabolic capabilities, including total denitrifying and grew at high temperatures capabilities. Culturable bacterial diversity in terrestrial subsurface habitats was represented by diverse chemoheterotrophic metabolic profiles. Bacterial strains from genera Beijerinckia, Agrobacterium, Pseudomonas, and Zobellella showed total denitrifying capability to N2 gaseous, while some strains from the genus Bacillus were thermophile bacteria. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences India Section B Biological Sciences Springer Journals

Characterization of a Bacterial Culture Collection from Terrestrial Subsurface Habitats in Colombia

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Springer Journals
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Copyright © The Author(s), under exclusive licence to The National Academy of Sciences, India 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
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0369-8211
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2250-1746
DOI
10.1007/s40011-023-01481-4
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Abstract

Microorganisms in terrestrial subsurface habitats have important roles in rock and mineral transformation and biogeochemical cycles processes. A group of 194 bacteria strains from three Colombian terrestrial subsurface habitats [oil fields (OF), coal mines (CM) and mud volcanoes (MV)] were characterized using both phylogenetic and phenotypic analyses. Hill´s number and related diversity indices were computed to know collection diversity and its relationship with sampling sites. Phylogenetic tree based on 16S rRNA gene dissimilarity values between strains showed seven major taxonomic/phylogenetic groups (α-, β-, δ-, γ-Proteobacteria, Actinobacteria, Clostridia and Bacilli) in culture collection, which contain sixty-one (61) putative bacteria species grouped in twenty-eight (28) genera. The widest represented genera of the culture collection were Bacillus, Beijerinckia, Cutibacterium, Desulfovibrio, Halomonas, Marinobacter, Pseudomonas, and Zobellella. The genus richness (q0) varied through sampling sites (MV > CM > OF). By the contrary, genus diversity based on relative abundances (q1) or relative abundances-evenness (q2) showed the follows diversity profiles: q1—(MV > OF > CM); q2—(MV > OF > CM). Genus’s abundances and evenness in overall collection resulted between moderate and high. The culture collection also showed bacterial groups with diverse metabolic capabilities, including total denitrifying and grew at high temperatures capabilities. Culturable bacterial diversity in terrestrial subsurface habitats was represented by diverse chemoheterotrophic metabolic profiles. Bacterial strains from genera Beijerinckia, Agrobacterium, Pseudomonas, and Zobellella showed total denitrifying capability to N2 gaseous, while some strains from the genus Bacillus were thermophile bacteria.

Journal

Proceedings of the National Academy of Sciences India Section B Biological Sciences – Springer Journals

Published: Jun 6, 2023

Keywords: Microbial culture collection; Terrestrial subsurface; 16S rRNA gene analysis; Denitrifying and thermophilic capabilities

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