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Characterization of Metal Tolerant Serratia spp. Isolates from Sediments of Uranium Ore Deposit of Domiasiat in Northeast India

Characterization of Metal Tolerant Serratia spp. Isolates from Sediments of Uranium Ore Deposit... Three metal tolerant Serratia spp. isolates from sediments of pre-mined uranium ore deposit of Domiasiat, Meghalaya, India were characterized using morphological, biochemical and molecular methods. 16S rRNA gene analysis of the isolates identified one of the isolates (DB-10) as Serratia nematodiphila with 99.33 % pairwise similarity whereas remaining two isolates (DB-11 and DB-15) showed similarity with Serratia marcescens subsp. sakuensis with similarity of 98.78 and 99.54 % respectively. Randomly amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) studies of the isolates revealed genetic diversity among them. The isolates were found to tolerate all the five tested metals namely, uranium, cadmium, copper, zinc and lead and also could resist nine commonly used antibiotics namely, ampicillin, kanamycin, chloramphenicol, erythromycin, gentamicin, aztreonam, tetracycline, imipenem and ciprofloxin. All the three isolates were found to be superior in tolerance and resistance to the reference strain S. marcescens ATCC13880. The isolates showed high efficiency of uranium (VI) removal ranging from 90 to 92 % (21.4–21.9 mg/L) and from 65 to 70 % (309.4–333.2 mg/L) when challenged with 100 μM (23.8 mg/L) and 2 mM (476 mg/L) uranyl nitrate solutions respectively within 1 h of incubation. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Characterization of Metal Tolerant Serratia spp. Isolates from Sediments of Uranium Ore Deposit of Domiasiat in Northeast India

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References (39)

Publisher
Springer Journals
Copyright
Copyright © 2013 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-013-0236-0
Publisher site
See Article on Publisher Site

Abstract

Three metal tolerant Serratia spp. isolates from sediments of pre-mined uranium ore deposit of Domiasiat, Meghalaya, India were characterized using morphological, biochemical and molecular methods. 16S rRNA gene analysis of the isolates identified one of the isolates (DB-10) as Serratia nematodiphila with 99.33 % pairwise similarity whereas remaining two isolates (DB-11 and DB-15) showed similarity with Serratia marcescens subsp. sakuensis with similarity of 98.78 and 99.54 % respectively. Randomly amplified polymorphic DNA (RAPD) and enterobacterial repetitive intergenic consensus sequence-based PCR (ERIC-PCR) studies of the isolates revealed genetic diversity among them. The isolates were found to tolerate all the five tested metals namely, uranium, cadmium, copper, zinc and lead and also could resist nine commonly used antibiotics namely, ampicillin, kanamycin, chloramphenicol, erythromycin, gentamicin, aztreonam, tetracycline, imipenem and ciprofloxin. All the three isolates were found to be superior in tolerance and resistance to the reference strain S. marcescens ATCC13880. The isolates showed high efficiency of uranium (VI) removal ranging from 90 to 92 % (21.4–21.9 mg/L) and from 65 to 70 % (309.4–333.2 mg/L) when challenged with 100 μM (23.8 mg/L) and 2 mM (476 mg/L) uranyl nitrate solutions respectively within 1 h of incubation.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Sep 24, 2013

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