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Characterization of polypropyleneimine as an alternative transfection reagent

Characterization of polypropyleneimine as an alternative transfection reagent Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.Graphical abstract[graphic not available: see fulltext] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Analytical Sciences Springer Journals

Characterization of polypropyleneimine as an alternative transfection reagent

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Publisher
Springer Journals
Copyright
Copyright © The Author(s), under exclusive licence to The Japan Society for Analytical Chemistry 2023. Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
ISSN
0910-6340
eISSN
1348-2246
DOI
10.1007/s44211-023-00284-x
Publisher site
See Article on Publisher Site

Abstract

Polypropyleneimine (PPI) was examined as a transfection reagent comparing with most widely used polymer, polyethyleneimine (PEI). PPI had better responsiveness to the endosomal pH and showed more condensation ability of plasmid DNA than PEI. Although the cytotoxicity of PPI was somewhat higher than PEI, the transfection efficacy of PPI was comparable with PEI or higher than PEI in some cell line. Thus, PPI would be an alternative transfection reagent.Graphical abstract[graphic not available: see fulltext]

Journal

Analytical Sciences – Springer Journals

Published: Jun 1, 2023

Keywords: Transfection; Polypropyleneimine; Synthetic vector

References