Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

Cytotoxicity and Oxidative Stress Alterations Induced by Aldrin in BALB/c 3T3 Fibroblast Cells

Cytotoxicity and Oxidative Stress Alterations Induced by Aldrin in BALB/c 3T3 Fibroblast Cells The intensive use of pesticides in agro-vet practices resulted in their persistence in environmental medium responsible for adverse effect on living system. The present study was planned to evaluate the cytotoxicity and oxidative stress potential of aldrin on BALB/c 3T3 mouse fibroblast cells. Short exposure of 3 h was given to the BALB/c 3T3 fibroblast cells under standard in vitro conditions. Inhibitory concentration-50 was estimated at the end of exposure period by neutral red uptake assay and was found to be 49.7 μg/ml. Further, cells were exposed with three concentrations of aldrin (12.4, 24.8 and 49.7 μg/ml) and 0.1 % dimethyl sulfoxide was used as negative control. Lipid peroxidation, antioxidant enzymes and non-enzymes were determined along with glucose-6-phosphate dehydrogenase, lactate dehydrogenase and alkaline phosphatase enzymes in BALB/c 3T3 fibroblast cells. Cells were also monitored for cell morphology during experiment. Exposure of aldrin to BALB/c 3T3 fibroblast cells resulted in increase in lipid peroxidation and decrease in antioxidant enzyme/non-enzyme system. Further, it caused decrease in glucose-6-phosphate dehydrogenase enzyme activity and excess leakage of lactate dehydrogenase and alkaline phosphatase enzymes into the medium. Aldrin-treated cells showed high degree of alteration in cell morphology indicating cell damage and death. These changes were noticed in dose dependent manner. In conclusion, the result of present study suggests that increase in lipid peroxidation and decrease in activity of antioxidant defence via oxidative stress by aldrin in BALB/c 3T3 cells were responsible for cytotoxicity. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Cytotoxicity and Oxidative Stress Alterations Induced by Aldrin in BALB/c 3T3 Fibroblast Cells

Download PDF
8 pages

Loading next page...
 
/lp/springer-journals/cytotoxicity-and-oxidative-stress-alterations-induced-by-aldrin-in-tY06933oPE

References (46)

Publisher
Springer Journals
Copyright
Copyright © 2016 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-015-0694-7
Publisher site
See Article on Publisher Site

Abstract

The intensive use of pesticides in agro-vet practices resulted in their persistence in environmental medium responsible for adverse effect on living system. The present study was planned to evaluate the cytotoxicity and oxidative stress potential of aldrin on BALB/c 3T3 mouse fibroblast cells. Short exposure of 3 h was given to the BALB/c 3T3 fibroblast cells under standard in vitro conditions. Inhibitory concentration-50 was estimated at the end of exposure period by neutral red uptake assay and was found to be 49.7 μg/ml. Further, cells were exposed with three concentrations of aldrin (12.4, 24.8 and 49.7 μg/ml) and 0.1 % dimethyl sulfoxide was used as negative control. Lipid peroxidation, antioxidant enzymes and non-enzymes were determined along with glucose-6-phosphate dehydrogenase, lactate dehydrogenase and alkaline phosphatase enzymes in BALB/c 3T3 fibroblast cells. Cells were also monitored for cell morphology during experiment. Exposure of aldrin to BALB/c 3T3 fibroblast cells resulted in increase in lipid peroxidation and decrease in antioxidant enzyme/non-enzyme system. Further, it caused decrease in glucose-6-phosphate dehydrogenase enzyme activity and excess leakage of lactate dehydrogenase and alkaline phosphatase enzymes into the medium. Aldrin-treated cells showed high degree of alteration in cell morphology indicating cell damage and death. These changes were noticed in dose dependent manner. In conclusion, the result of present study suggests that increase in lipid peroxidation and decrease in activity of antioxidant defence via oxidative stress by aldrin in BALB/c 3T3 cells were responsible for cytotoxicity.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Jan 8, 2016

There are no references for this article.