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Damage-induced cell–cell communication in different cochlear cell types via two distinct ATP-dependent Ca2+ waves

Damage-induced cell–cell communication in different cochlear cell types via two distinct... Intercellular Ca2+ waves can coordinate the action of large numbers of cells over significant distances. Recent work in many different systems has indicated that the release of ATP is fundamental for the propagation of most Ca2+ waves. In the organ of hearing, the cochlea, ATP release is involved in critical signalling events during tissue maturation. ATP-dependent signalling is also implicated in the normal hearing process and in sensing cochlear damage. Here, we show that two distinct Ca2+ waves are triggered during damage to cochlear explants. Both Ca2+ waves are elicited by extracellular ATP acting on P2 receptors, but they differ in their source of Ca2+, their velocity, their extent of spread and the cell type through which they propagate. A slower Ca2+ wave (14 μm/s) communicates between Deiters’ cells and is mediated by P2Y receptors and Ca2+ release from IP3-sensitive stores. In contrast, a faster Ca2+ wave (41 μm/s) propagates through sensory hair cells and is mediated by Ca2+ influx from the external environment. Using inhibitors and selective agonists of P2 receptors, we suggest that the faster Ca2+ wave is mediated by P2X4 receptors. Thus, in complex tissues, the expression of different receptors determines the propagation of distinct intercellular communication signals. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Purinergic Signalling Springer Journals

Damage-induced cell–cell communication in different cochlear cell types via two distinct ATP-dependent Ca2+ waves

Lahne, Manuela; Gale, Jonathan
Purinergic Signalling , Volume 6 (2) – Jul 6, 2010
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References (47)

Publisher
Springer Journals
Copyright
Copyright © 2010 by Springer Science+Business Media B.V.
Subject
Biomedicine; Cancer Research ; Neurosciences ; Human Physiology ; Pharmacology/Toxicology ; Biomedicine general
ISSN
1573-9538
eISSN
1573-9546
DOI
10.1007/s11302-010-9193-8
pmid
20806011
Publisher site
See Article on Publisher Site

Abstract

Intercellular Ca2+ waves can coordinate the action of large numbers of cells over significant distances. Recent work in many different systems has indicated that the release of ATP is fundamental for the propagation of most Ca2+ waves. In the organ of hearing, the cochlea, ATP release is involved in critical signalling events during tissue maturation. ATP-dependent signalling is also implicated in the normal hearing process and in sensing cochlear damage. Here, we show that two distinct Ca2+ waves are triggered during damage to cochlear explants. Both Ca2+ waves are elicited by extracellular ATP acting on P2 receptors, but they differ in their source of Ca2+, their velocity, their extent of spread and the cell type through which they propagate. A slower Ca2+ wave (14 μm/s) communicates between Deiters’ cells and is mediated by P2Y receptors and Ca2+ release from IP3-sensitive stores. In contrast, a faster Ca2+ wave (41 μm/s) propagates through sensory hair cells and is mediated by Ca2+ influx from the external environment. Using inhibitors and selective agonists of P2 receptors, we suggest that the faster Ca2+ wave is mediated by P2X4 receptors. Thus, in complex tissues, the expression of different receptors determines the propagation of distinct intercellular communication signals.

Journal

Purinergic Signalling – Springer Journals

Published: Jul 6, 2010

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