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Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India

Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae... Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. https://doi.org/10.1007/s40011-017-0960-y RESEARCH ARTICLE Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India 1,2 3 2 4 • • • • V. Venkataravanappa P. Swarnalatha Sujoy Saha C. N. Lakshminarayana Reddy M. Krishna Reddy Received: 27 February 2017 / Revised: 19 December 2017 / Accepted: 27 December 2017 The National Academy of Sciences, India 2018 Abstract Tomato plants showing witches broom symp- TBB-Vns showed similarity coefficient of 0.68–0.95. Since toms were collected from different states of India. The threshold similarity coefficient for classifying the phyto- presence of phytoplasma infection was confirmed by PCR plasma into new subgroup is set at 0.97, the strain under using phytoplasma-specific primer of 16S rRNA and SecY study significantly distinct from the representative strains gene. The sequence analysis of 16S rRNA and SecY gene in the subgroups of pea nut witches broom. Further, the of eight tomato big bud phytoplasmas showed maximum phylogenetic analysis of tomato big bud phytoplasmas nucleotide (nt) identity of 95–100% with Peanut WB group revealed that, they are closely clustered with peanut wit- (16SrII). Further in-silico RFLP analysis of 16S rRNA ches’-broom strains http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India

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References (28)

Publisher
Springer Journals
Copyright
Copyright © 2018 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-017-0960-y
Publisher site
See Article on Publisher Site

Abstract

Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. https://doi.org/10.1007/s40011-017-0960-y RESEARCH ARTICLE Detection, Characterization and In-Silico Analysis of Candidatus Phytoplasma australasiae Associated with Big Bud Disease of Tomato in India 1,2 3 2 4 • • • • V. Venkataravanappa P. Swarnalatha Sujoy Saha C. N. Lakshminarayana Reddy M. Krishna Reddy Received: 27 February 2017 / Revised: 19 December 2017 / Accepted: 27 December 2017 The National Academy of Sciences, India 2018 Abstract Tomato plants showing witches broom symp- TBB-Vns showed similarity coefficient of 0.68–0.95. Since toms were collected from different states of India. The threshold similarity coefficient for classifying the phyto- presence of phytoplasma infection was confirmed by PCR plasma into new subgroup is set at 0.97, the strain under using phytoplasma-specific primer of 16S rRNA and SecY study significantly distinct from the representative strains gene. The sequence analysis of 16S rRNA and SecY gene in the subgroups of pea nut witches broom. Further, the of eight tomato big bud phytoplasmas showed maximum phylogenetic analysis of tomato big bud phytoplasmas nucleotide (nt) identity of 95–100% with Peanut WB group revealed that, they are closely clustered with peanut wit- (16SrII). Further in-silico RFLP analysis of 16S rRNA ches’-broom strains

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Jan 12, 2018

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