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Development of Recombinant Matrix Metalloproteinase-11 Based Sandwich ELISA for the Diagnosis of Canine Mammary Tumor

Development of Recombinant Matrix Metalloproteinase-11 Based Sandwich ELISA for the Diagnosis of... Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbits and guinea pigs to standardize sandwich ELISA (sELISA) for relative quantification of circulating MMP-11 for the diagnosis of canine mammary tumor (n = 50). The test was found to be 98 % sensitive and 92 % specific at receiver-operating characteristics (ROC) with respect to histopathological staining in diagnosing canine mammary tumors. The area under the ROC curve (AUC) of rMMP-11 based sELISA, which represented a summary statistics of the overall diagnostic performance of the test also indicated that it could be used as a suitable test for diagnosing mammary neoplasms. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Development of Recombinant Matrix Metalloproteinase-11 Based Sandwich ELISA for the Diagnosis of Canine Mammary Tumor

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References (18)

Publisher
Springer Journals
Copyright
Copyright © 2012 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-012-0127-9
Publisher site
See Article on Publisher Site

Abstract

Certain matrix metalloproteinases like MMP-11 (Stromelysin-3) and MMP-7 (Matrilysin) are reported to be highly expressed in breast cancer, so they may act as marker enzymes for breast cancer progression. Hyperimmune sera were raised against heterologously expressed recombinant canine MMP-11 in rabbits and guinea pigs to standardize sandwich ELISA (sELISA) for relative quantification of circulating MMP-11 for the diagnosis of canine mammary tumor (n = 50). The test was found to be 98 % sensitive and 92 % specific at receiver-operating characteristics (ROC) with respect to histopathological staining in diagnosing canine mammary tumors. The area under the ROC curve (AUC) of rMMP-11 based sELISA, which represented a summary statistics of the overall diagnostic performance of the test also indicated that it could be used as a suitable test for diagnosing mammary neoplasms.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Nov 25, 2012

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