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DNA and RNA Isolation Techniques for Non-ExpertsDensity Gradient-Based Nucleic Acid Isolation

DNA and RNA Isolation Techniques for Non-Experts: Density Gradient-Based Nucleic Acid Isolation [Nucleic acid (DNA/RNA) isolation has a wide range of applications, right from research purposes to applications such as food development, identification of the new organism and their categorization, diagnosing patients with genetic mutations, and also the expression levels of specific genes. Therefore, the scientific fraternity has devised new and innovative methods for the isolation of the said biomolecules. However, although a little old, the density gradient method is yet being used in many laboratories and yields decent results. The method employs a very simple principle in which the components are separated on the basis of their density with respect to the solvent used. Cesium chloride (CsCl) is one of the popular solvents used in the density gradient method. Under the influence of high centrifugal force, a solution of cesium chloride (CsCl) molecules will dissociate, leading to the formation of a narrow density gradient. The nucleic acid in question will migrate in this gradient to the point with a similar density (the neutral buoyancy or isopycnic point). This present article gives a detailed view of the isolation of the nucleic acids using the density gradient method. It highlights the different steps and the precautions one needs to observe while working on the said experiment.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsDensity Gradient-Based Nucleic Acid Isolation

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References (1)

  • RH Burdon (1988)

    18

    Laboratory Techniques in Biochemistry and Molecular Biology Elsevier, 18

Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
119 –123
DOI
10.1007/978-3-030-94230-4_16
Publisher site
See Chapter on Publisher Site

Abstract

[Nucleic acid (DNA/RNA) isolation has a wide range of applications, right from research purposes to applications such as food development, identification of the new organism and their categorization, diagnosing patients with genetic mutations, and also the expression levels of specific genes. Therefore, the scientific fraternity has devised new and innovative methods for the isolation of the said biomolecules. However, although a little old, the density gradient method is yet being used in many laboratories and yields decent results. The method employs a very simple principle in which the components are separated on the basis of their density with respect to the solvent used. Cesium chloride (CsCl) is one of the popular solvents used in the density gradient method. Under the influence of high centrifugal force, a solution of cesium chloride (CsCl) molecules will dissociate, leading to the formation of a narrow density gradient. The nucleic acid in question will migrate in this gradient to the point with a similar density (the neutral buoyancy or isopycnic point). This present article gives a detailed view of the isolation of the nucleic acids using the density gradient method. It highlights the different steps and the precautions one needs to observe while working on the said experiment.]

Published: Mar 30, 2022

Keywords: Nucleic acids; Deoxyribonucleic acid; Ribonucleic acid; Density; Cesium chloride

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