Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

DNA and RNA Isolation Techniques for Non-ExpertsDNA Extraction by Spooling Method

DNA and RNA Isolation Techniques for Non-Experts: DNA Extraction by Spooling Method [A typical mammalian cell contains about six picograms and 2.2 m of DNA molecule. Therefore, the isolation of whole DNA from any human organ consisting of around 200 billion cells will be challenging and tedious. But the extraction of DNA by spooling method helps the experimenter isolate such a large quantity and long fibers of DNA conveniently. The nonexperts, in particular, can visualize the white translucent DNA fibers after spooling in this technique. The basic protocol consists of cell lysis and selective precipitation of DNA using different biochemicals. This is followed by the rapid spooling of precipitated DNA fibers on the glass rod or stirring rod under cold temperature conditions. Though the obtained DNA is free from proteins and other impurities, further processing of this DNA might be required for specific molecular biological applications.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsDNA Extraction by Spooling Method

Download PDF
3 pages

Loading next page...
 
/lp/springer-journals/dna-and-rna-isolation-techniques-for-non-experts-dna-extraction-by-uEY0t53iFj

References (2)

  • J Sambrook (2006)

    pdb-prot4037

    Cold Spring Harbor Protocols, 2006

  • K Nath (1990)

    6462

    Nucleic Acids Research, 18

Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
107 –110
DOI
10.1007/978-3-030-94230-4_14
Publisher site
See Chapter on Publisher Site

Abstract

[A typical mammalian cell contains about six picograms and 2.2 m of DNA molecule. Therefore, the isolation of whole DNA from any human organ consisting of around 200 billion cells will be challenging and tedious. But the extraction of DNA by spooling method helps the experimenter isolate such a large quantity and long fibers of DNA conveniently. The nonexperts, in particular, can visualize the white translucent DNA fibers after spooling in this technique. The basic protocol consists of cell lysis and selective precipitation of DNA using different biochemicals. This is followed by the rapid spooling of precipitated DNA fibers on the glass rod or stirring rod under cold temperature conditions. Though the obtained DNA is free from proteins and other impurities, further processing of this DNA might be required for specific molecular biological applications.]

Published: Mar 30, 2022

Keywords: DNA; Spooling; Fibers; Mammalian cells; RNAse

There are no references for this article.