Get 20M+ Full-Text Papers For Less Than $1.50/day. Start a 14-Day Trial for You or Your Team.

Learn More →

DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Lysozyme and Proteinase K

DNA and RNA Isolation Techniques for Non-Experts: DNA Isolation by Lysozyme and Proteinase K [The isolation of DNA using lysozyme and Proteinase K is suitable for extraction of DNA from microorganisms particularly Gram-positive bacteria. The application of enzymes like lysozyme and Proteinase K at suitable temperatures, duration, and pH helps in the degradation of hard and rigid peptidoglycan cell walls of these microorganisms. Therefore, it is the most efficient and quick method for the isolation of DNA from microorganisms without using harsh biochemicals. However, further purification of DNA can be required by other methods for utilizing it further for molecular studies. This method is widely used in microbiology research, production of recombinant DNAs, biotechnology-based industries, and medical diagnosis where the presence of microbial DNA confirms the pathogenicity of a particular disease.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Lysozyme and Proteinase K

Download PDF
3 pages

Loading next page...
 
/lp/springer-journals/dna-and-rna-isolation-techniques-for-non-experts-dna-isolation-by-0O7R4XE65F

References (2)

  • C Bollet (1991)

    1955

    Nucleic Acids Research, 19

  • R Martzy (2019)

    1

    Scientific Reports, 9

Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
85 –88
DOI
10.1007/978-3-030-94230-4_11
Publisher site
See Chapter on Publisher Site

Abstract

[The isolation of DNA using lysozyme and Proteinase K is suitable for extraction of DNA from microorganisms particularly Gram-positive bacteria. The application of enzymes like lysozyme and Proteinase K at suitable temperatures, duration, and pH helps in the degradation of hard and rigid peptidoglycan cell walls of these microorganisms. Therefore, it is the most efficient and quick method for the isolation of DNA from microorganisms without using harsh biochemicals. However, further purification of DNA can be required by other methods for utilizing it further for molecular studies. This method is widely used in microbiology research, production of recombinant DNAs, biotechnology-based industries, and medical diagnosis where the presence of microbial DNA confirms the pathogenicity of a particular disease.]

Published: Mar 30, 2022

Keywords: DNA; Microorganisms; Gram-positive bacteria; Proteinase K; Lysozyme

There are no references for this article.