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DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Chelex Method

DNA and RNA Isolation Techniques for Non-Experts: DNA Isolation by Chelex Method [The Chelex method of DNA extraction is suitable for extracting the DNA from a smaller amount of samples. This method is quick and straightforward and does not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH 10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to denature. Resin beads bind to the cellular components, while DNA (and RNA) remains dissolved in the aqueous solution. Chelex resin also inhibits DNA degradation by chelating metal ions. This method is particularly beneficial for forensic applications, but it is not appropriate for large-scale DNA extraction. The resulting single-stranded DNA is less stable, therefore, not suitable for long-term storage and RFLP analysis.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsDNA Isolation by Chelex Method

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
79 –84
DOI
10.1007/978-3-030-94230-4_10
Publisher site
See Chapter on Publisher Site

Abstract

[The Chelex method of DNA extraction is suitable for extracting the DNA from a smaller amount of samples. This method is quick and straightforward and does not involve any harmful organic solvents. The basic protocol involves the extraction of DNA by adding samples to hot Chelex suspensions at pH 10–11. The alkalinity of resin suspension and exposure to heat result in disruption of the cell membrane. Heating also causes the double helix of DNA to denature. Resin beads bind to the cellular components, while DNA (and RNA) remains dissolved in the aqueous solution. Chelex resin also inhibits DNA degradation by chelating metal ions. This method is particularly beneficial for forensic applications, but it is not appropriate for large-scale DNA extraction. The resulting single-stranded DNA is less stable, therefore, not suitable for long-term storage and RFLP analysis.]

Published: Mar 30, 2022

Keywords: Chelex 100; Forensic science; Pathology; Chelating resin; PCR

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