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DNA and RNA Isolation Techniques for Non-ExpertsIsolation of DNA from Blood Samples by Salting Method

DNA and RNA Isolation Techniques for Non-Experts: Isolation of DNA from Blood Samples by Salting... [Extraction of blood genomic DNA is a systematic method in clinical and molecular biology research. Despite the fact that several procedures for extracting blood genomic DNA have been developed, the bulk of these processes are time-consuming and involve expensive chemicals as well as dangerous organic solvents. The majority of the materials used in standard DNA extraction are hazardous, and it is desirable to adopt a successful DNA extraction process that does not need such chemicals. For genetic research, high-quality and high-quantity DNA extraction is essential. It is critical to address the usage of easy and low-cost DNA extraction technologies in large-scale gene polymorphism investigations. This chapter aims to examine the most successful way of extracting DNA using the salt-out method. This method is easy, quick, safe, and inexpensive. It may be utilized in medical laboratories and research facilities.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsIsolation of DNA from Blood Samples by Salting Method

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Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
89 –93
DOI
10.1007/978-3-030-94230-4_12
Publisher site
See Chapter on Publisher Site

Abstract

[Extraction of blood genomic DNA is a systematic method in clinical and molecular biology research. Despite the fact that several procedures for extracting blood genomic DNA have been developed, the bulk of these processes are time-consuming and involve expensive chemicals as well as dangerous organic solvents. The majority of the materials used in standard DNA extraction are hazardous, and it is desirable to adopt a successful DNA extraction process that does not need such chemicals. For genetic research, high-quality and high-quantity DNA extraction is essential. It is critical to address the usage of easy and low-cost DNA extraction technologies in large-scale gene polymorphism investigations. This chapter aims to examine the most successful way of extracting DNA using the salt-out method. This method is easy, quick, safe, and inexpensive. It may be utilized in medical laboratories and research facilities.]

Published: Mar 30, 2022

Keywords: DNA isolation; Salting-out method; Cell lysis; Genomic DNA

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