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DNA and RNA Isolation Techniques for Non-ExpertsTransformation or Genetic Modification of Cells/Organisms

DNA and RNA Isolation Techniques for Non-Experts: Transformation or Genetic Modification of... [Genetic engineering technology has been employed for a long time to enhance the quantity and quality of a product and produce the desired product from an organism. As a critical biotechnological tool, genetic engineering and modifications have solved problems like food security, biofuel production, etc. Additionally, these methods have also been employed to design novel biological systems by modifying the existing ones by either introducing transgenes or reconstructing the present genetic makeup. This chapter describes the different methods like nuclear transformation, biolistic transformation, electroporation, sonoporation, etc., which have been employed to manipulate the genetic constitution of organisms and applications of these methods.] http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png

DNA and RNA Isolation Techniques for Non-ExpertsTransformation or Genetic Modification of Cells/Organisms

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References (4)

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  • Y Du (2019)

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  • A Bihon Asfaw (2019)

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    Cogent Food & Agriculture, 5

  • M El Karoui (2019)

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    Frontiers in Bioengineering and Biotechnology, 7

Publisher
Springer International Publishing
Copyright
© The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Nature Switzerland AG 2022
ISBN
978-3-030-94229-8
Pages
135 –145
DOI
10.1007/978-3-030-94230-4_18
Publisher site
See Chapter on Publisher Site

Abstract

[Genetic engineering technology has been employed for a long time to enhance the quantity and quality of a product and produce the desired product from an organism. As a critical biotechnological tool, genetic engineering and modifications have solved problems like food security, biofuel production, etc. Additionally, these methods have also been employed to design novel biological systems by modifying the existing ones by either introducing transgenes or reconstructing the present genetic makeup. This chapter describes the different methods like nuclear transformation, biolistic transformation, electroporation, sonoporation, etc., which have been employed to manipulate the genetic constitution of organisms and applications of these methods.]

Published: Mar 30, 2022

Keywords: Genetic engineering; Nuclear transformation; Biolistic transformation; Conjugation; Transduction

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