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DNA from Buccal Swabs Recruited by Mail: Evaluation of Storage Effects on Long-Term Stability and Suitability for Multiplex Polymerase Chain Reaction Genotyping

DNA from Buccal Swabs Recruited by Mail: Evaluation of Storage Effects on Long-Term Stability and... We provide details of an inexpensive and rapid method for extraction of DNA from buccal swabs (including samples received through the mail) and from a range of other tissue samples. The procedure we have developed provides amounts of DNA adequate for several thousand polymerase chain reactions (PCRs), and we have validated its potential for long-term storage. Samples stored for >4 years are of comparable concentration and provide as robust PCR templates as those tested immediately after extraction. The availability of this technology is of considerable significance in planning DNA banks from population collections and cohorts. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Behavior Genetics Springer Journals

DNA from Buccal Swabs Recruited by Mail: Evaluation of Storage Effects on Long-Term Stability and Suitability for Multiplex Polymerase Chain Reaction Genotyping

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References (7)

Publisher
Springer Journals
Copyright
Copyright © 2003 by Plenum Publishing Corporation
Subject
Biomedicine; Human Genetics; Neurosciences; Evolutionary Biology
ISSN
0001-8244
eISSN
1573-3297
DOI
10.1023/A:1021055617738
Publisher site
See Article on Publisher Site

Abstract

We provide details of an inexpensive and rapid method for extraction of DNA from buccal swabs (including samples received through the mail) and from a range of other tissue samples. The procedure we have developed provides amounts of DNA adequate for several thousand polymerase chain reactions (PCRs), and we have validated its potential for long-term storage. Samples stored for >4 years are of comparable concentration and provide as robust PCR templates as those tested immediately after extraction. The availability of this technology is of considerable significance in planning DNA banks from population collections and cohorts.

Journal

Behavior GeneticsSpringer Journals

Published: Oct 6, 2004

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