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Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic Characteristics in Indian Tomato Genotypes

Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic... Random Amplified Polymorphic DNA (RAPD) technique is one of the simplest marker techniques used in the differentiation of organisms. In the present study, 28 Indian tomato genotypes along with morphological, biochemical and ionomic characteristics were subjected to RAPD analysis. Ten random primers were selected from 100 operon primers which produced 88 reproducible bands; 89.8 % (79) of bands were polymorphic and 10.2 % (9) were monomorphic. The total number of markers identified by SMA in different parameters were plant height-06, fruit number-07, fruit cluster-03, fruit weight-03, pH-03, titratable acidity-03, TSS-03, total sugars-07, reducing sugars-06, non-reducing sugars-02, and lycopene-05. In case of macroelements, the total number of markers identified in SMA was P-03, K-05, Ca-06, Mg-03; for microelements, Mn-03, Fe-01, Co-06, Ni-06, Cu-04, Bo-06, Na-04, Mo-07, Cd-02 and Li-06. The total markers associated by SMRA showed the following. Plant height-06, fruit number-11, fruit cluster-06, fruit weight-17, pH-03, titratable acidity-04, TSS-04, total sugars-19, reducing sugars-20, non-reducing sugars-14, lycopene-19, P-13, K-18, Ca-03, Mg-03, Iron-13, Co-04, Ni-13, Cu-16, Zn-26, Bo-22, Na-21, Mo-20, Cd-18 and Li-12. The study suggested that the RAPD markers identified could be used in breeding elite tomato genotypes with good characteristics. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Identification of RAPD Markers Associated with Morphological, Biochemical and Ionomic Characteristics in Indian Tomato Genotypes

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References (26)

Publisher
Springer Journals
Copyright
Copyright © 2013 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-013-0202-x
Publisher site
See Article on Publisher Site

Abstract

Random Amplified Polymorphic DNA (RAPD) technique is one of the simplest marker techniques used in the differentiation of organisms. In the present study, 28 Indian tomato genotypes along with morphological, biochemical and ionomic characteristics were subjected to RAPD analysis. Ten random primers were selected from 100 operon primers which produced 88 reproducible bands; 89.8 % (79) of bands were polymorphic and 10.2 % (9) were monomorphic. The total number of markers identified by SMA in different parameters were plant height-06, fruit number-07, fruit cluster-03, fruit weight-03, pH-03, titratable acidity-03, TSS-03, total sugars-07, reducing sugars-06, non-reducing sugars-02, and lycopene-05. In case of macroelements, the total number of markers identified in SMA was P-03, K-05, Ca-06, Mg-03; for microelements, Mn-03, Fe-01, Co-06, Ni-06, Cu-04, Bo-06, Na-04, Mo-07, Cd-02 and Li-06. The total markers associated by SMRA showed the following. Plant height-06, fruit number-11, fruit cluster-06, fruit weight-17, pH-03, titratable acidity-04, TSS-04, total sugars-19, reducing sugars-20, non-reducing sugars-14, lycopene-19, P-13, K-18, Ca-03, Mg-03, Iron-13, Co-04, Ni-13, Cu-16, Zn-26, Bo-22, Na-21, Mo-20, Cd-18 and Li-12. The study suggested that the RAPD markers identified could be used in breeding elite tomato genotypes with good characteristics.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Jul 24, 2013

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