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Identification of Rice Blast Resistance Gene Pi9 from Indian Rice Land Races with STS Marker and Its Verification by Virulence Analysis

Identification of Rice Blast Resistance Gene Pi9 from Indian Rice Land Races with STS Marker and... The Pi9 gene in rice confers resistance to strains of blast pathogen Magnaporthe oryzae. Pi9 is a typical broad spectrum resistance gene containing nucleotide binding site, leucine rich repeat family of sequences. In the present study, presence of the Pi9 gene in 47 rice germplasm accessions was determined using dominant sequence tagged site marker 195R-1/195F-1 derived from the Nbs2-Pi9 candidate gene and resistance confirmed by inoculating rice germplasm with a mixture of aggressive isolates of M. oryzae namely Mo-ei-66, Mo-ei-79, Mo-ei-119, and Mo-ei-202. The Pi9 gene was found in six rice germplasm accessions from eastern India. Usefulness of this STS marker for determination of the genotype of rice germplasm was thus demonstrated. Rare occurrence of Pi9 gene in the evaluated rice germplasm suggests that its introgression is very less in indica rice. These results are useful for incorporating Pi9 gene into elite cultivars by marker assisted selection in rice breeding programs worldwide. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Identification of Rice Blast Resistance Gene Pi9 from Indian Rice Land Races with STS Marker and Its Verification by Virulence Analysis

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References (53)

Publisher
Springer Journals
Copyright
Copyright © 2013 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-013-0186-6
Publisher site
See Article on Publisher Site

Abstract

The Pi9 gene in rice confers resistance to strains of blast pathogen Magnaporthe oryzae. Pi9 is a typical broad spectrum resistance gene containing nucleotide binding site, leucine rich repeat family of sequences. In the present study, presence of the Pi9 gene in 47 rice germplasm accessions was determined using dominant sequence tagged site marker 195R-1/195F-1 derived from the Nbs2-Pi9 candidate gene and resistance confirmed by inoculating rice germplasm with a mixture of aggressive isolates of M. oryzae namely Mo-ei-66, Mo-ei-79, Mo-ei-119, and Mo-ei-202. The Pi9 gene was found in six rice germplasm accessions from eastern India. Usefulness of this STS marker for determination of the genotype of rice germplasm was thus demonstrated. Rare occurrence of Pi9 gene in the evaluated rice germplasm suggests that its introgression is very less in indica rice. These results are useful for incorporating Pi9 gene into elite cultivars by marker assisted selection in rice breeding programs worldwide.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological SciencesSpringer Journals

Published: May 8, 2013

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