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The Plant Book
- Publisher
- Springer Journals
- Copyright
- Copyright © 2014 by The National Academy of Sciences, India
- Subject
- Life Sciences; Life Sciences, general; Behavioral Sciences; Plant Biochemistry; Nucleic Acid Chemistry
- ISSN
- 0369-8211
- eISSN
- 2250-1746
- DOI
- 10.1007/s40011-014-0420-x
- Publisher site
- See Article on Publisher Site
Abstract
An in vitro micropropagation system has been developed for the Ceropegia anjanerica Malpure et al., a rare endemic plant species having ornamental as well as medicinal potential but a limited reproductive capacity. Axillary bud explants were cultured on Murashige and Skoog medium supplemented with 6-Benzylaminopurine, Thidiazuron and 6-Furfurylaminopurine along with combination of Indole-3-Butyric Acid. Although all concentrations showed multiple shoot formation, the best results (8.0 ± 0.5) were achieved with MS + 6-Benzylaminopurine (8.87 µM) + 6-Furfurylaminopurine (18.58 µM). Half strength Murashige and Skoog medium fortified with Indole-3-Acetic Acid (8.56 µM) showed good rooting response (4.0 ± 0.7). Well grown plantlets hardened, acclimatized and established in greenhouse exhibited 78 % survival.
Journal
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: Dec 4, 2014