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Induction of Apoptosis in Glioblastoma Cell Line (U87-MG) by Caspian Cobra (Naja naja oxiana) Snake Venom

Induction of Apoptosis in Glioblastoma Cell Line (U87-MG) by Caspian Cobra (Naja naja oxiana)... Due to drug resistance, efforts are ongoing to develop new anticancer agent especially from the screening of natural compounds. The current study aimed to evaluate the cytotoxic effects of crude venom of Caspian cobra (Naja naja oxiana, NNO) in glioblastoma cell line, U87-MG cells. After 24 h exposure to the venom at the concentrations of 5, 20, and 40 µg/ml, the percentage of cells undergoing apoptosis and necrosis were evaluated by flow cytometry analysis. Gene expression of bax and bcl-2 were also measured by real-time polymerase chain reaction. The findings showed that venom of NNO is cytotoxic against U87-MG cells through dose-dependent increase in the percentage of apoptotic cells and bax gene expression. Furthermore, venom of NNO induced necrosis at the high dose of 40 µg/ml. The results suggest that NNO venom may initiate the intrinsic pathway of apoptosis in the glioblastoma cancer cell line by affection of the mitochondrial pathways. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png "Proceedings of the National Academy of Sciences, India Section B:Biological Sciences" Springer Journals

Induction of Apoptosis in Glioblastoma Cell Line (U87-MG) by Caspian Cobra (Naja naja oxiana) Snake Venom

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References (31)

Publisher
Springer Journals
Copyright
Copyright © The National Academy of Sciences, India 2022
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-021-01332-0
Publisher site
See Article on Publisher Site

Abstract

Due to drug resistance, efforts are ongoing to develop new anticancer agent especially from the screening of natural compounds. The current study aimed to evaluate the cytotoxic effects of crude venom of Caspian cobra (Naja naja oxiana, NNO) in glioblastoma cell line, U87-MG cells. After 24 h exposure to the venom at the concentrations of 5, 20, and 40 µg/ml, the percentage of cells undergoing apoptosis and necrosis were evaluated by flow cytometry analysis. Gene expression of bax and bcl-2 were also measured by real-time polymerase chain reaction. The findings showed that venom of NNO is cytotoxic against U87-MG cells through dose-dependent increase in the percentage of apoptotic cells and bax gene expression. Furthermore, venom of NNO induced necrosis at the high dose of 40 µg/ml. The results suggest that NNO venom may initiate the intrinsic pathway of apoptosis in the glioblastoma cancer cell line by affection of the mitochondrial pathways.

Journal

"Proceedings of the National Academy of Sciences, India Section B:Biological Sciences" – Springer Journals

Published: Jun 1, 2022

Keywords: Caspian cobra venom; Glioblastoma multiforme; U87-MG; Apoptosis; Cytotoxicity

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