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Inhibition of KSHV-associated lymphoma engraftment in SCID mouse by morpholino oligomers

Inhibition of KSHV-associated lymphoma engraftment in SCID mouse by morpholino oligomers Nan et al. Infectious Agents and Cancer 2010, 5(Suppl 1):A40 http://www.infectagentscancer.com/content/5/S1/A40 MEETING ABSTRACTS Open Access Inhibition of KSHV-associated lymphoma engraftment in SCID mouse by morpholino oligomers Yuchen Nan , Deendayal Patel, Yanjin Zhang th From 12 International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI) Bethesda, MD, USA. 26-27 April, 2010 Immunodeficiencies (ICMAOI). The full contents of the supplement are Kaposi’s sarcoma-associated herpesvirus (KSHV), also available online at http://www.biomedcentral.com/1750-9378/5?issue=S1. known as human herpesvirus 8, is associated with sev- eral malignant disorders, including Kaposi’ssarcoma, Published: 11 October 2010 primary effusion lymphoma (PEL), and multicentric Cas- tleman’s disease. We have explored peptide-conjugated antisense phosphorodiamidate morpholino oligonucleo- doi:10.1186/1750-9378-5-S1-A40 Cite this article as: Nan et al.: Inhibition of KSHV-associated lymphoma tides (PPMOs) against KSHV and found effective engraftment in SCID mouse by morpholino oligomers. Infectious Agents PPMOs in inhibition of KSHV gene expression in cell and Cancer 2010 5(Suppl 1):A40. culture. PPMOs are single-stranded DNA analogues that have a modified backbone and penetrate cells readily. In this study, we further tested the PPMOs in a SCID mouse model to assess their effect on engraftment and growth of PEL cells. PEL cells were engrafted into SCID mice via intraperitoneal route. PPMO was administered at the same time and repeated every other day for 10 doses. The mice were observed and scored for ascites development. The tumor cell burden was assessed by flow cytometry. Administration of anti-vIL-6 PPMO protected the mice from lymphoma development, while those mice receiving a control PPMO developed ascites and had high ratio of PEL cells in peritoneal lavage. The results demonstrate that PPMO against key KSHV genes can potently reduce KSHV replication and growth of PEL cells in SCID mice. Further exploration of PPMOs Submit your next manuscript to BioMed Central in the animal model is warranted. and take full advantage of: • Convenient online submission Acknowledgements This article has been published as part of Infectious Agents and Cancer • Thorough peer review th Volume 5 Supplement 1, 2010: Proceedings of the 12 International • No space constraints or color figure charges Conference on Malignancies in AIDS and Other Acquired • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution * Correspondence: zhangyj@umd.edu Department of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA Submit your manuscript at www.biomedcentral.com/submit © 2010 Nan et al; licensee BioMed Central Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Infectious Agents and Cancer Springer Journals

Inhibition of KSHV-associated lymphoma engraftment in SCID mouse by morpholino oligomers

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Nan et al; licensee BioMed Central Ltd.
Subject
Biomedicine; Cancer Research; Infectious Diseases; Oncology
eISSN
1750-9378
DOI
10.1186/1750-9378-5-S1-A40
Publisher site
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Abstract

Nan et al. Infectious Agents and Cancer 2010, 5(Suppl 1):A40 http://www.infectagentscancer.com/content/5/S1/A40 MEETING ABSTRACTS Open Access Inhibition of KSHV-associated lymphoma engraftment in SCID mouse by morpholino oligomers Yuchen Nan , Deendayal Patel, Yanjin Zhang th From 12 International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI) Bethesda, MD, USA. 26-27 April, 2010 Immunodeficiencies (ICMAOI). The full contents of the supplement are Kaposi’s sarcoma-associated herpesvirus (KSHV), also available online at http://www.biomedcentral.com/1750-9378/5?issue=S1. known as human herpesvirus 8, is associated with sev- eral malignant disorders, including Kaposi’ssarcoma, Published: 11 October 2010 primary effusion lymphoma (PEL), and multicentric Cas- tleman’s disease. We have explored peptide-conjugated antisense phosphorodiamidate morpholino oligonucleo- doi:10.1186/1750-9378-5-S1-A40 Cite this article as: Nan et al.: Inhibition of KSHV-associated lymphoma tides (PPMOs) against KSHV and found effective engraftment in SCID mouse by morpholino oligomers. Infectious Agents PPMOs in inhibition of KSHV gene expression in cell and Cancer 2010 5(Suppl 1):A40. culture. PPMOs are single-stranded DNA analogues that have a modified backbone and penetrate cells readily. In this study, we further tested the PPMOs in a SCID mouse model to assess their effect on engraftment and growth of PEL cells. PEL cells were engrafted into SCID mice via intraperitoneal route. PPMO was administered at the same time and repeated every other day for 10 doses. The mice were observed and scored for ascites development. The tumor cell burden was assessed by flow cytometry. Administration of anti-vIL-6 PPMO protected the mice from lymphoma development, while those mice receiving a control PPMO developed ascites and had high ratio of PEL cells in peritoneal lavage. The results demonstrate that PPMO against key KSHV genes can potently reduce KSHV replication and growth of PEL cells in SCID mice. Further exploration of PPMOs Submit your next manuscript to BioMed Central in the animal model is warranted. and take full advantage of: • Convenient online submission Acknowledgements This article has been published as part of Infectious Agents and Cancer • Thorough peer review th Volume 5 Supplement 1, 2010: Proceedings of the 12 International • No space constraints or color figure charges Conference on Malignancies in AIDS and Other Acquired • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution * Correspondence: zhangyj@umd.edu Department of Veterinary Medicine and Maryland Pathogen Research Institute, University of Maryland, College Park, MD, USA Submit your manuscript at www.biomedcentral.com/submit © 2010 Nan et al; licensee BioMed Central Ltd.

Journal

Infectious Agents and Cancer – Springer Journals

Published: Oct 11, 2010

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