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Isolation, Purification and Partial Characterization of an Antibacterial Agent Produced by Halotolerant Alkaliphilic Streptomyces sp. EWC 7(2)

Isolation, Purification and Partial Characterization of an Antibacterial Agent Produced by... A halotolerant alkaliphilic actinomycetes strain EWC 7(2) was isolated from earthworm castings located in Dehradun, India. It showed the morphological, chemotaxonomic and physiological characteristics of the genus Streptomyces. On the basis of 16S rDNA sequence analysis and physiological tests, this isolate was found to be most closely related with Streptomyces ghanaensis. The optimum metabolite production was recorded in Glucose soyabean meal medium which is active against both Gram-positive and Gram-negative bacteria including drug resistant bacterial pathogens. The antibacterial activity started in the beginning of the exponential phase of growth (2 days) and reached maximum at 3–8 days while the maximum dry mycelial weight was obtained by 3–5 days of fermentation. The best growth and metabolite production was observed at a salt concentration of 4 % (w/v) and above pH 8. The antibiotic was extracted with HP Dianion resin eluted with methanol and detected by bioautography on silica gel plates using Micrococcus luteus MTCC106 as test organism. The results of bioautography revealed the presence of single active compound. The antibiotic compound was further purified by semi-prep HPLC. The spectroscopic studies such as UV–Vis, infrared, and mass of the compound showed that the molecule may be different from other known antibiotics produced by most closely related species of Streptomyces EWC 7(2). The results obtained from this study are promising however; further studies are required to elucidate the structure of the active compound. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Isolation, Purification and Partial Characterization of an Antibacterial Agent Produced by Halotolerant Alkaliphilic Streptomyces sp. EWC 7(2)

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References (48)

Publisher
Springer Journals
Copyright
Copyright © 2012 by The National Academy of Sciences, India
Subject
Life Sciences; Life Sciences, general; Behavioural Sciences; Plant Biochemistry; Nucleic Acid Chemistry
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-012-0117-y
Publisher site
See Article on Publisher Site

Abstract

A halotolerant alkaliphilic actinomycetes strain EWC 7(2) was isolated from earthworm castings located in Dehradun, India. It showed the morphological, chemotaxonomic and physiological characteristics of the genus Streptomyces. On the basis of 16S rDNA sequence analysis and physiological tests, this isolate was found to be most closely related with Streptomyces ghanaensis. The optimum metabolite production was recorded in Glucose soyabean meal medium which is active against both Gram-positive and Gram-negative bacteria including drug resistant bacterial pathogens. The antibacterial activity started in the beginning of the exponential phase of growth (2 days) and reached maximum at 3–8 days while the maximum dry mycelial weight was obtained by 3–5 days of fermentation. The best growth and metabolite production was observed at a salt concentration of 4 % (w/v) and above pH 8. The antibiotic was extracted with HP Dianion resin eluted with methanol and detected by bioautography on silica gel plates using Micrococcus luteus MTCC106 as test organism. The results of bioautography revealed the presence of single active compound. The antibiotic compound was further purified by semi-prep HPLC. The spectroscopic studies such as UV–Vis, infrared, and mass of the compound showed that the molecule may be different from other known antibiotics produced by most closely related species of Streptomyces EWC 7(2). The results obtained from this study are promising however; further studies are required to elucidate the structure of the active compound.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Oct 30, 2012

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