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MicroRNA analysis in human papillomavirus (HPV)-associated cervical neoplasia and cancer

MicroRNA analysis in human papillomavirus (HPV)-associated cervical neoplasia and cancer Gardiner et al. Infectious Agents and Cancer 2010, 5(Suppl 1):A55 http://www.infectagentscancer.com/content/5/S1/A55 MEETING ABSTRACTS Open Access MicroRNA analysis in human papillomavirus (HPV)-associated cervical neoplasia and cancer 1* 2 2 2 2 3 Amy S Gardiner , William C McBee Jr. , Robert P Edwards , Marshall Austin , Jamie L Lesnock , Rohit Bhargava , 2 1 Richard Guido , Saleem A Khan th From 12 International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI) Bethesda, MD, USA. 26-27 April, 2010 Author details MicroRNAs (miRNAs) are ~22 nt single-stranded, non- Department of Microbiology and Molecular Genetics, University of protein-coding RNAs that generally negatively regulate Pittsburgh School of Medicine, Pittsburgh, PA, USA. Division of Gynecologic their target mRNAs at a posttranscriptional level. Differ- Oncology, Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Women’s Hospital of the University of Pittsburgh Medical ential expression of miRNAs has been observed in many Center, Pittsburgh, PA, USA. Department of Pathology, Magee-Women’s human cancers. To study their potential role in the Hospital of the University of Pittsburgh Medical Center, Pittsburgh, PA, USA. pathogenesis of human papillomavirus (HPV) type 16- Published: 11 October 2010 associated cervical neoplasia and cancer, we analyzed miRNA expression in cervical tissue from the normal cervix, moderate/severe dysplasia, and invasive squa- doi:10.1186/1750-9378-5-S1-A55 mous cell carcinoma. Using RNA from 6 cervical can- Cite this article as: Gardiner et al.: MicroRNA analysis in human cers, 3 dysplasias, and 4 normal samples and the papillomavirus (HPV)-associated cervical neoplasia and cancer. Infectious TaqMan® MicroRNA Arrays, we found that 18 miRNAs Agents and Cancer 2010 5(Suppl 1):A55. were overexpressed and 2 underexpressed in cervical cancer compared to the normal cervical tissue (p<0.05). We further found that 9 miRNAs (miRs-16, 21, 106b, 124, 135b, 223, 301b, 449a, and 141) were consistently overexpressed and 2 miRNAs (miRs-218 and 433) were consistently underexpressed in cervical cancer compared to the normal tissue. MiRNA expression in dysplasia samples was most similar to the normal tissue, with the exception of the overexpression of miR-16, miR-141, and miR-449a, and the underexpression of miR-218 and miR-433. Our results suggest that five miRNAs may have potential as markers for progression of dysplasia to invasive cervical disease. Submit your next manuscript to BioMed Central and take full advantage of: Acknowledgements This article has been published as part of Infectious Agents and Cancer • Convenient online submission th Volume 5 Supplement 1, 2010: Proceedings of the 12 International • Thorough peer review Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI). The full contents of the supplement are • No space constraints or color figure charges available online at http://www.biomedcentral.com/1750-9378/5?issue=S1. • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar *Correspondence: asg18@pitt.edu 1 • Research which is freely available for redistribution Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA Full list of author information is available at the end of the article Submit your manuscript at www.biomedcentral.com/submit © 2010 Gardiner et al; licensee BioMed Central Ltd. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Infectious Agents and Cancer Springer Journals

MicroRNA analysis in human papillomavirus (HPV)-associated cervical neoplasia and cancer

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Publisher
Springer Journals
Copyright
Copyright © 2010 by Gardiner et al; licensee BioMed Central Ltd.
Subject
Biomedicine; Cancer Research; Infectious Diseases; Oncology
eISSN
1750-9378
DOI
10.1186/1750-9378-5-S1-A55
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Abstract

Gardiner et al. Infectious Agents and Cancer 2010, 5(Suppl 1):A55 http://www.infectagentscancer.com/content/5/S1/A55 MEETING ABSTRACTS Open Access MicroRNA analysis in human papillomavirus (HPV)-associated cervical neoplasia and cancer 1* 2 2 2 2 3 Amy S Gardiner , William C McBee Jr. , Robert P Edwards , Marshall Austin , Jamie L Lesnock , Rohit Bhargava , 2 1 Richard Guido , Saleem A Khan th From 12 International Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI) Bethesda, MD, USA. 26-27 April, 2010 Author details MicroRNAs (miRNAs) are ~22 nt single-stranded, non- Department of Microbiology and Molecular Genetics, University of protein-coding RNAs that generally negatively regulate Pittsburgh School of Medicine, Pittsburgh, PA, USA. Division of Gynecologic their target mRNAs at a posttranscriptional level. Differ- Oncology, Department of Obstetrics, Gynecology, and Reproductive Sciences, Magee-Women’s Hospital of the University of Pittsburgh Medical ential expression of miRNAs has been observed in many Center, Pittsburgh, PA, USA. Department of Pathology, Magee-Women’s human cancers. To study their potential role in the Hospital of the University of Pittsburgh Medical Center, Pittsburgh, PA, USA. pathogenesis of human papillomavirus (HPV) type 16- Published: 11 October 2010 associated cervical neoplasia and cancer, we analyzed miRNA expression in cervical tissue from the normal cervix, moderate/severe dysplasia, and invasive squa- doi:10.1186/1750-9378-5-S1-A55 mous cell carcinoma. Using RNA from 6 cervical can- Cite this article as: Gardiner et al.: MicroRNA analysis in human cers, 3 dysplasias, and 4 normal samples and the papillomavirus (HPV)-associated cervical neoplasia and cancer. Infectious TaqMan® MicroRNA Arrays, we found that 18 miRNAs Agents and Cancer 2010 5(Suppl 1):A55. were overexpressed and 2 underexpressed in cervical cancer compared to the normal cervical tissue (p<0.05). We further found that 9 miRNAs (miRs-16, 21, 106b, 124, 135b, 223, 301b, 449a, and 141) were consistently overexpressed and 2 miRNAs (miRs-218 and 433) were consistently underexpressed in cervical cancer compared to the normal tissue. MiRNA expression in dysplasia samples was most similar to the normal tissue, with the exception of the overexpression of miR-16, miR-141, and miR-449a, and the underexpression of miR-218 and miR-433. Our results suggest that five miRNAs may have potential as markers for progression of dysplasia to invasive cervical disease. Submit your next manuscript to BioMed Central and take full advantage of: Acknowledgements This article has been published as part of Infectious Agents and Cancer • Convenient online submission th Volume 5 Supplement 1, 2010: Proceedings of the 12 International • Thorough peer review Conference on Malignancies in AIDS and Other Acquired Immunodeficiencies (ICMAOI). The full contents of the supplement are • No space constraints or color figure charges available online at http://www.biomedcentral.com/1750-9378/5?issue=S1. • Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar *Correspondence: asg18@pitt.edu 1 • Research which is freely available for redistribution Department of Microbiology and Molecular Genetics, University of Pittsburgh School of Medicine, Pittsburgh, PA, USA Full list of author information is available at the end of the article Submit your manuscript at www.biomedcentral.com/submit © 2010 Gardiner et al; licensee BioMed Central Ltd.

Journal

Infectious Agents and Cancer – Springer Journals

Published: Oct 11, 2010

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