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Modeling of Optimal Conditions for Immobilized Glucose Oxidase in Novel Polymeric Matrix Synthesized by Molecularly Imprinting Technique

Modeling of Optimal Conditions for Immobilized Glucose Oxidase in Novel Polymeric Matrix... As most enzymatic biocatalysts are soluble in water, effort for separation of free enzyme from the reaction media is one of the main challenges in industrial application. Therefore, in recent years, enzyme immobilization methods have been drawn attention due to the easier separation and possible reutilization of enzyme. In this survey, a novel immobilization technique was employed for entrapment of glucose oxidase enzyme using molecularly imprinted polymers, and the optimal conditions in both free and immobilized enzymes were investigated by response surface methodology and central composite design. For modeling and optimization of enzymatic activity, five main variables including substrate (4–9 mM) and enzyme concentrations (1–2 mg/L), oxygen rate (1–2 L/min), pH (5–7), and temperature (30–40 °C) were employed as independent factors by the use of Design Expert software. The maximum amount of enzyme activity was achieved equal to 1578 and 1306 μmol/min for free and immobilized enzyme, respectively. Therefore, it can be concluded that produced immobilized enzyme with high activity can be used for various industrial applications because of the easier separation and possible reutilization of enzyme. http://www.deepdyve.com/assets/images/DeepDyve-Logo-lg.png Proceedings of the National Academy of Sciences, India Section B: Biological Sciences Springer Journals

Modeling of Optimal Conditions for Immobilized Glucose Oxidase in Novel Polymeric Matrix Synthesized by Molecularly Imprinting Technique

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References (32)

Publisher
Springer Journals
Copyright
Copyright © The National Academy of Sciences, India 2021
ISSN
0369-8211
eISSN
2250-1746
DOI
10.1007/s40011-021-01250-1
Publisher site
See Article on Publisher Site

Abstract

As most enzymatic biocatalysts are soluble in water, effort for separation of free enzyme from the reaction media is one of the main challenges in industrial application. Therefore, in recent years, enzyme immobilization methods have been drawn attention due to the easier separation and possible reutilization of enzyme. In this survey, a novel immobilization technique was employed for entrapment of glucose oxidase enzyme using molecularly imprinted polymers, and the optimal conditions in both free and immobilized enzymes were investigated by response surface methodology and central composite design. For modeling and optimization of enzymatic activity, five main variables including substrate (4–9 mM) and enzyme concentrations (1–2 mg/L), oxygen rate (1–2 L/min), pH (5–7), and temperature (30–40 °C) were employed as independent factors by the use of Design Expert software. The maximum amount of enzyme activity was achieved equal to 1578 and 1306 μmol/min for free and immobilized enzyme, respectively. Therefore, it can be concluded that produced immobilized enzyme with high activity can be used for various industrial applications because of the easier separation and possible reutilization of enzyme.

Journal

Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals

Published: Sep 1, 2021

Keywords: Biocatalyst; Glucose oxidase; Immobilized enzyme; Molecularly imprinting technique; Statistical modeling

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