Access the full text.
Sign up today, get DeepDyve free for 14 days.
K. Bowman, U. Albrecht, J. Graham, D. Bright (2007)Detection of Phytophthora nicotianae and P. palmivora in citrus roots using PCR-RFLP in comparison with other methods
European Journal of Plant Pathology, 119
(1996)Studies on the sporulation of the early blight agent of tomatoes
B. Manicom, M. Bar-joseph, A. Rosner, H. Vigodsky-Haas, J. Kotzé (1987)Potential applications of random DNA probes and restriction fragment length polymorphisms in the taxonomy of the fusaria.
J. Sambrook, E. Fritsch, T. Maniatis (2001)Molecular Cloning: A Laboratory Manual
Hong-kai Wang, Tianyu Zhang, Meng Zhang (2001)Application of sequencing of 5.8S rDNA, ITS1 and ITS2 on identification and classification of Alternaria at sepecies level
M. Naik, Y. Prasad, K. Bhat, G. Rani (2010)Morphological, physiological, pathogenic and molecular variability among isolates of Alternaria solani from tomato
Indian phytopathology, 63
K. Tamura, G. Stecher, D. Peterson, A. Filipski, Sudhir Kumar (2013)MEGA6: Molecular Evolutionary Genetics Analysis version 6.0.
Molecular biology and evolution, 30 12
R. Vunsh, A. Rosner, A. Stein (1990)The use of the polymerase chain reaction (PCR) for the detection of bean yellow mosaic virus in gladiolus.
Annals of Applied Biology, 117
V. Datar, C. Mayee (1981)Assessment of losses in tomato yield due to early blight
SMDTP Gomes, EDB Carneiro, EP Romano, MZ Teixeira, ME Costa, JCG Vasconcelos (2010)Effect of biotherapic of Alternaria solani on the early blight of tomato-plant and the in vitro development of the fungus
Int J High Dilution Res, 9
P. Bridge, T. Singh, D. Arora (2004)The application of molecular markers in the epidemiology of plant pathogenic fungi.
K. Pandey, P. Pandey, G. Kalloo, M. Banerjee (2003)Resistance to early blight of tomato with respect to various parameters of disease epidemics
Journal of General Plant Pathology, 69
Khalaf Alhussa (2012)Morphological and Physiological Characterization of Alternaria solani Isolated from Tomato in Jordan Valley
Research Journal of Biological Sciences, 7
A. Derbalah, M. El-Mahrouk, A. El-Sayed (2011)Efficacy and Safety of Some Plant Extracts against Tomato Early Blight Disease Caused by Alternaria solani
Plant Pathology Journal, 10
B. Pryor, R. Gilbertson (2000)Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences
Fungal Biology, 104
J. Bhatt, A. Gahlain, S. Pant (2000)Record of Alternaria alternata on tomato, capsicum and spinach in Kumaon hills
Indian phytopathology, 53
K. Abd-Elsalam (2003)Non-gel based techniques for plant pathogen genotyping.
Acta microbiologica Polonica, 52 4
J. Thompson, D. Higgins, T. Gibson (1994)CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice.
Nucleic acids research, 22 22
Liang-Dong Guo, Li Xu, W. Zheng, K. Hyde (2004)Genetic variation of Alternaria alternata, an endophytic fungus isolated from Pinus tabulaeformis as determined by random amplified microsatelites (RAMS)
LD Guo, L Xu, WH Zheng, KD Hyde (2004)Genetic variation of Alternaria
Fungal Divers, 16
T. White (1990)Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics
B. Pryor, T. Michailides (2002)Morphological, pathogenic, and molecular characterization of alternaria isolates associated with alternaria late blight of pistachio.
Phytopathology, 92 4
R. Radhajeyalakshmi, R. Velazhahan, R. Samiyappan, S. Doraiswamy (2009)Systemic induction of pathogenesis related proteins (PRs) in Alternaria solani elicitor sensitized tomato cells as resistance response.
Scientific Research and Essays, 4
KA Abd-Esalem, A Asran-Amel, F Schneider, Q Migheli, JA Verreet (2006)Molecular detection of Fusarium oxysporum. f. sp. vasinfectum in cotton roots by PCR and real-time PCR assay
J Plant Dis Prot, 113
Virendra Kumar, S. Haldar, K. Pandey, R. Singh, A. Singh, Prabhash Singh (2008)Cultural, morphological, pathogenic and molecular variability amongst tomato isolates of Alternaria solani in India
World Journal of Microbiology and Biotechnology, 24
M. Kusaba, T. Tsuge (1995)Phologeny of Alternaria fungi known to produce host-specific toxins on the basis of variation in internal transcribed spacers of ribosomal DNA
Current Genetics, 28
P. Varma, Sherjugjit Singh, S. Gandhi (2007)Variability among Alternaria solani isolates causing early blight of tomato
Indian phytopathology, 60
BM Pryor, RL Gilbertson (2000)Molecular phylogenetic relationships amongst Alternaria species and related fungi based upon analysis of nuclear ITS and mt SSU rDNA sequences
Mycol Res, 104
M. Innis, D. Gelfand, J. Sninsky, T. White (1990)PCR protocols: a guide to methods and applications.
MK Naik, Y Prasad, KV Bhat, GS Devika Rani (2010)Morphological, physiological, pathogenic and molecular variability among isolates of Alternaria
Indian Phytopathol, 63
D. Arora (2003)Fungal Biotechnology in Agricultural, Food, and Environmental Applications
Early blight disease samples were collected from six different states of India. Seventeen different isolates of Alternaria spp were obtained in pure culture which were designated as PB-2, PB-4, PB-13, PB-17, PB-19, HR-12, HR-44, HR-62, MP-6, MP-7, JT-4, JT-11, UP-7, UP-10, UP-15, UP-20 and RJ-T-1. Characterization based on culture colour and conidial dimensions (conidial length and breadth, and beak length) indicated that no distinguished variation of the isolates was observed on the colour parameters. Although, a wide variation was observed in the length of conidia and beak, no significant difference was observed in conidial breadth except RJ-T-1. Among the isolates, conidial length of five isolates viz., HR-12, UP-7, UP-10, UP-15 and RJ-T-1 ranged from 80 to 97 µm whereas others were of significantly lesser size (30–51 µm). Similarly, significant difference was observed in beak length. The PCR amplification of the fungal DNA using universal primers ITS1 and ITS4 and sequencing indicated that, out of 17 isolates, 12 (JT-4, JT-11, HR-62, HR-44, PB-2, PB-4, PB-13, PB-17, PB-19, UP-20, MP-6 and MP-7) were closely matching with A. alternata (JX993756) and five isolates (HR-12, UP-7, UP-10, UP-15 and RJ-T-1) with A. solani (JF796068). Further, pathogenicity test on tomato revealed that both A. alternata and A. solani isolates were of virulent category indicating that former is also an incitant of early blight in northern India.
Proceedings of the National Academy of Sciences, India Section B: Biological Sciences – Springer Journals
Published: Oct 29, 2014
Access the full text.
Sign up today, get DeepDyve free for 14 days.